Department of Pharmacy, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan.
Molecules. 2011 Sep 28;16(10):8181-97. doi: 10.3390/molecules16108181.
Two new urethane-based acrylates (UAA and PEG-UAA) were synthesized as polymer blocks. The chemical composition of the two monomers was confirmed by IR and NMR. After cross-linking these blockers by radical polymerization, "hexamethylene PU" [poly(hexamethylene-urethane)] and "PEG-hexamethylene PU" [PEG-poly(hexa-methylene-urethane)] were obtained. The platelet adhesion and platelet activation of these polymers were evaluated in the presence of Platelet Rich Plasma (PRP) blood. The relative blood clotting indexes of the polymers were determined to measure their capability of reducing thrombogenicity. The hemolysis of red blood cells was also assessed to examine the haemocompatibility of the polymers. The hexamethylene PU and PEG-hexamethylene PU showed less platelet adhesion, platelet activation, blood clotting and hemolysis than a commercial PU (Tecoflex). The liquid crystal molecule, cholesteryl oleyl carbonate (COC), showed further improved biocompatibility to human blood, after COC was embedded in the PU polymers. PEG-hexamethylene PU + 10% COC demonstrated the best activity in reducing thrombogenicity and the best haemocompatibility. The inclusion of PEG segments into the PEG-UAA structure increased its hydrophilicity. The methylene bis(cyclohexyl) segments in Tecoflex PU decreased haemocompatibility. These observations are in good agreement with performed contact angle measurements. The PEG-hexamethylene PU loaded with COC might be a promising material for applications in bioengineering.
两种新型的基于氨酯的丙烯酸酯（UAA 和 PEG-UAA）被合成作为聚合物链段。两种单体的化学组成通过 IR 和 NMR 得到确认。通过自由基聚合交联这些阻断剂后，得到了“六亚甲基聚氨酯”[聚（六亚甲基异氰酸酯）]和“PEG-六亚甲基聚氨酯”[PEG-聚（六亚甲基异氰酸酯）]。在富含血小板的血浆（PRP）血液存在的情况下，评估了这些聚合物的血小板黏附和血小板激活。测定聚合物的相对凝血指数以测量其降低血栓形成性的能力。还评估了红细胞溶血以检查聚合物的血液相容性。与商业聚氨酯（Tecoflex）相比，六亚甲基聚氨酯和 PEG-六亚甲基聚氨酯显示出较少的血小板黏附、血小板激活、血液凝固和溶血。液晶分子胆甾醇辛基碳酸酯（COC）在嵌入 PU 聚合物后，对人血显示出进一步改善的生物相容性。PEG-六亚甲基聚氨酯+10%COC 在降低血栓形成性和改善血液相容性方面表现最佳。PEG-UAA 结构中 PEG 段的加入增加了其亲水性。Tecoflex PU 中的亚甲基双（环己基）片段降低了血液相容性。这些观察结果与进行的接触角测量结果一致。负载 COC 的 PEG-六亚甲基聚氨酯可能是生物工程应用中有前途的材料。
