Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Vet Rec. 2010 Sep 4;167(10):376-8. doi: 10.1136/vr.c3748.
The objective of this study was to detect and characterise the biovar of equine herpesvirus type 1 (EHV-1) from submandibular lymph nodes (SMLNs) and trigeminal ganglia from 153 equids undergoing routine postmortem examination for various medical and surgical reasons. A combination of nucleic acid precipitation and preamplification steps was used to increase the analytical sensitivity of the analysis. The presence of latent EHV-1 was determined when tissue samples were PCR-positive for the glycoprotein B (gB) gene and the DNA polymerase (ORF 30) gene of EHV-1 in the absence of detectable late structural protein gene (gB gene) mRNA. The SMLNs of five of the study animals (3.3 per cent) were PCR-positive for the gB gene of EHV-1. Two SMLNs carried a latent neurotropic strain of the virus, whereas three SMLNs were PCR-positive for both neurotropic and non-neurotropic EHV-1. A total of 30 trigeminal ganglia collected from 19 horses were PCR-positive for the gB gene of EHV-1. Nine trigeminal ganglia harboured either latent non-neurotropic or neurotropic EHV-1 strains. Twelve trigeminal ganglia contained both latent neurotropic and non-neurotropic EHV-1. The prevalence and distribution of EHV-1 biovars among the study horses appeared to be influenced by their breed and the type of tissue tested.
本研究的目的是从因各种医疗和外科原因进行常规尸检的 153 匹马的颌下淋巴结（SMLN）和三叉神经节中检测和描述马疱疹病毒 1 型（EHV-1）的生物型。采用核酸沉淀和预扩增步骤的组合来提高分析的灵敏度。当组织样本的 PCR 对 EHV-1 的糖蛋白 B（gB）基因和 EHV-1 的 DNA 聚合酶（ORF 30）基因呈阳性，而没有检测到可检测的晚期结构蛋白基因（gB 基因）mRNA 时，确定潜伏的 EHV-1 的存在。研究动物中有 5 只（3.3%）的 SMLN 的 EHV-1 gB 基因 PCR 呈阳性。两个 SMLN 携带潜伏的神经毒力株病毒，而三个 SMLN 对神经毒力和非神经毒力的 EHV-1 均呈阳性。从 19 匹马中收集的总共 30 个三叉神经节的 EHV-1 gB 基因 PCR 呈阳性。9 个三叉神经节含有潜伏的非神经毒力或神经毒力 EHV-1 株。12 个三叉神经节含有潜伏的神经毒力和非神经毒力的 EHV-1。研究马匹中 EHV-1 生物型的流行率和分布似乎受到其品种和测试的组织类型的影响。
