Leese H J, Lenton E A
Department of Biology, University of York, Heslington, UK.
Hum Reprod. 1990 Nov;5(8):915-9. doi: 10.1093/oxfordjournals.humrep.a137219.
The concentrations of glucose, lactate, progesterone and oestradiol have been measured in 122 follicular fluids obtained from women attending a natural cycle IVF programme. Some women received low-dose clomiphene. Fluids were recovered 28-35 h after the onset of the spontaneous LH surge. Mean follicular fluid volumes were greater for stimulated (4.46 ml) than spontaneous (3.11 ml) cycles. Mean glucose and lactate concentrations were similar in the two groups, with overall means of 3.29 mM (glucose) and 6.12 mM (lactate). Total glucose and lactate were greater in the fluids from stimulated (14.1 and 29.5 mol, respectively) than from the spontaneous cycles (11.5 and 20.2 mmol, respectively). There were negative correlations between follicular fluid volume and glucose concentration (r = -0.348) and between glucose concentration and lactate concentration (r = -0.367) but no relationship between follicular fluid volume and lactate concentration (r = 0.086). A model is presented to account for these findings in terms of the movement of pyruvate, glucose and lactate from the vascular theca into follicular fluid, and of glycolysis occurring in the granulosa cells.
对参加自然周期体外受精计划的女性所获取的122份卵泡液中的葡萄糖、乳酸、孕酮和雌二醇浓度进行了测量。部分女性接受了低剂量克罗米芬治疗。在自发促黄体生成素峰出现后28 - 35小时收集卵泡液。刺激周期（4.46毫升）的平均卵泡液体积大于自然周期（3.11毫升）。两组的平均葡萄糖和乳酸浓度相似，总体平均值分别为3.29毫摩尔/升（葡萄糖）和6.12毫摩尔/升（乳酸）。刺激周期卵泡液中的总葡萄糖和乳酸含量（分别为14.1和29.5微摩尔）高于自然周期（分别为11.5和20.2毫摩尔）。卵泡液体积与葡萄糖浓度之间（r = -0.348）以及葡萄糖浓度与乳酸浓度之间（r = -0.367）呈负相关，但卵泡液体积与乳酸浓度之间无相关性（r = 0.086）。本文提出了一个模型，用以解释丙酮酸、葡萄糖和乳酸从血管膜向卵泡液的移动以及颗粒细胞中发生的糖酵解过程所导致的这些研究结果。
