Department of Applied Physics, Royal Institute of Technology, AlbaNova University Center, SE-106 91, Stockholm, Sweden.
Lab Chip. 2010 Oct 21;10(20):2727-32. doi: 10.1039/c004707d. Epub 2010 Aug 31.
We demonstrate a microplate platform for parallelized manipulation of particles or cells by frequency-modulated ultrasound. The device, consisting of a silicon-glass microchip and a single ultrasonic transducer, enables aggregation, positioning and high-resolution microscopy of cells distributed in an array of 100 microwells centered on the microchip. We characterize the system in terms of temperature control, aggregation and positioning efficiency, and cell viability. We use time-lapse imaging to show that cells continuously exposed to ultrasound are able to divide and remain viable for at least 12 hours inside the device. Thus, the device can be used to induce and maintain aggregation in a parallelized fashion, facilitating long-term microscopy studies of, e.g., cell-cell interactions.
我们展示了一种基于调频超声的微流控芯片平台，用于并行操作粒子或细胞。该装置由硅玻璃微芯片和单个超声换能器组成，能够实现位于微芯片中心的 100 个微孔阵列中细胞的聚集、定位和高分辨率显微镜观察。我们从温度控制、聚集和定位效率以及细胞活力等方面对系统进行了表征。我们使用延时成像表明，持续暴露于超声下的细胞能够分裂，并在设备内至少存活 12 小时。因此，该设备可用于以并行方式诱导和维持聚集，从而便于进行细胞间相互作用等长期的显微镜研究。
