Department of Neurosurgery, Tainan Sin-Lau Hospital, Tainan 71101, Taiwan.
J Agric Food Chem. 2010 Oct 13;58(19):10639-45. doi: 10.1021/jf1016303.
Curcuminoids, natural plant components, have been recently shown to display antioxidant and anti-inflammatory activities. They also produce potent chemo-preventive action against several types of cancer. In the present study, the anti-proliferative and induced apoptosis effects of curcuminoids have been investigated in human brain glioblastoma multiforme (GBM) 8401 cells. Results indicated that curcuminoids have produced an inhibition of cell proliferation in a dose-dependent manner as dosage increased from 12.5 to 100 μM (n = 6) via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay as well as activation of apoptosis in GBM 8401 cells. Both effects were observed to increase in proportion with the dose of curcuminoids. We have studied the mitochondrial membrane potential (ΔΨm), DNA fragmentation, caspase-3, caspase-8, and caspase-9 activation, and nuclear factor κB (NF-κB) transcriptional factor activity to analyze apoptosis in GBM 8401 cells. From these approaches, apoptosis was induced by curcuminoids in human brain GBM 8401 cells via mitochondria and a caspase-dependent pathway. The results observed with proliferation inhibition (y = 94.694e(-0.025x), R(2) = 0.9901, and n = 6) and apoptosis (y = 0.9789e(-0.0102x), R(2) = 0.99854, and n = 3) depend upon the amount of curcuminoid treatment in the cancer cells.
姜黄素类，天然植物成分，最近被证明具有抗氧化和抗炎活性。它们还对多种癌症产生强大的化学预防作用。在本研究中，研究了姜黄素类对人脑胶质母细胞瘤（GBM）8401 细胞的抗增殖和诱导细胞凋亡作用。结果表明，姜黄素类通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴盐（MTT）测定法，剂量从 12.5 增加到 100 μM（n = 6），剂量依赖性地抑制细胞增殖，以及在 GBM 8401 细胞中激活细胞凋亡。这两种作用都随着姜黄素类剂量的增加而增加。我们研究了线粒体膜电位（ΔΨm）、DNA 片段化、caspase-3、caspase-8 和 caspase-9 的激活以及核因子 κB（NF-κB）转录因子活性，以分析 GBM 8401 细胞中的细胞凋亡。从这些方法中，姜黄素类通过线粒体和 caspase 依赖性途径诱导人脑 GBM 8401 细胞凋亡。观察到的增殖抑制（y = 94.694e(-0.025x)，R(2) = 0.9901，n = 6）和凋亡（y = 0.9789e(-0.0102x)，R(2) = 0.99854，n = 3）取决于癌细胞中姜黄素类的处理量。
