Chakravarti Bulbul, Mallik Buddhadeb, Chakravarti Deb N
Department of Biology and Department of Chemistry, York College, City University of New York, New York, CA, USA.
Methods Mol Biol. 2010;662:3-28. doi: 10.1007/978-1-60761-800-3_1.
Studies of complex biological systems aimed at understanding their functions at a global level are the goals of systems biology. Proteomics, generally regarded as the comprehensive study of the expression of all the proteins at a particular time in different organs, tissues, and cell types is a key enabling technology for the systems biology approach. Rapid advances in this regard have been made following the success of the human genome project as well as those of various animals and microorganisms. Possibly, one of the most promising outcomes from studies on the human genome and proteome is the identification of potential new drugs for the treatment of different diseases and tailoring the drugs for individualized patient therapy. Following the identification of a new drug candidate, knowledge on organ and system-level responses helps prioritize the drug targets and design clinical trials based on their efficacy and safety. Toxicoproteomics is playing an important role in that respect. In essence, over the past decade, proteomics has played a major role in drug discovery and development. In this review article, we explain systems biology, discuss the current proteomic technologies, and highlight some important applications of proteomics and systems biology approaches in drug discovery and development.
旨在从整体层面理解其功能的复杂生物系统研究是系统生物学的目标。蛋白质组学通常被视为对特定时间不同器官、组织和细胞类型中所有蛋白质表达的全面研究，是系统生物学方法的一项关键支撑技术。随着人类基因组计划以及各种动物和微生物基因组计划的成功，在这方面已取得了迅速进展。也许，人类基因组和蛋白质组研究最有前景的成果之一是识别出治疗不同疾病的潜在新药，并为个体化患者治疗量身定制药物。在识别出新药候选物之后，关于器官和系统水平反应的知识有助于确定药物靶点的优先级，并根据其疗效和安全性设计临床试验。毒理蛋白质组学在这方面正发挥着重要作用。从本质上讲，在过去十年中，蛋白质组学在药物发现和开发中发挥了重要作用。在这篇综述文章中，我们解释了系统生物学，讨论了当前的蛋白质组学技术，并重点介绍了蛋白质组学和系统生物学方法在药物发现和开发中的一些重要应用。
