Department of Food Science and Technology, Graphic Era (Deemed to Be) University, Bell Road, Clement Town, Dehradun 248002, Uttarakhand, India.
Himalayan School of Biosciences, Swami Rama Himalayan University, Swami Rama Nagar, Jolly Grant, Dehradun 248014, Uttarakhand, India.
Int J Mol Sci. 2023 Feb 3;24(3):3025. doi: 10.3390/ijms24033025.
To explore changes in proteins and metabolites under stress circumstances, genomics, proteomics, and metabolomics methods are used. In-depth research over the previous ten years has gradually revealed the fundamental processes of plants' responses to environmental stress. Abiotic stresses, which include temperature extremes, water scarcity, and metal toxicity brought on by human activity and urbanization, are a major cause for concern, since they can result in unsustainable warming trends and drastically lower crop yields. Furthermore, there is an emerging reliance on agrochemicals. Stress is responsible for physiological transformations such as the formation of reactive oxygen, stomatal opening and closure, cytosolic calcium ion concentrations, metabolite profiles and their dynamic changes, expression of stress-responsive genes, activation of potassium channels, etc. Research regarding abiotic stresses is lacking because defense feedbacks to abiotic factors necessitate regulating the changes that activate multiple genes and pathways that are not properly explored. It is clear from the involvement of these genes that plant stress response and adaptation are complicated processes. Targeting the multigenicity of plant abiotic stress responses caused by genomic sequences, transcripts, protein organization and interactions, stress-specific and cellular transcriptome collections, and mutant screens can be the first step in an integrative approach. Therefore, in this review, we focused on the genomes, proteomics, and metabolomics of tomatoes under abiotic stress.
为了探究应激条件下蛋白质和代谢物的变化,人们采用了基因组学、蛋白质组学和代谢组学方法。在过去十年的深入研究中,植物对环境胁迫的反应的基本过程逐渐被揭示出来。非生物胁迫,包括由人类活动和城市化引起的极端温度、缺水和金属毒性,是一个主要的关注点,因为它们可能导致不可持续的变暖趋势和作物产量的大幅下降。此外,人们越来越依赖农用化学品。胁迫导致生理转化,如活性氧的形成、气孔的开启和关闭、胞质钙离子浓度、代谢物谱及其动态变化、应激响应基因的表达、钾通道的激活等。由于对非生物因子的防御反馈需要调节激活多个基因和途径的变化,而这些基因和途径尚未得到充分探索,因此非生物胁迫的研究还存在不足。这些基因的参与表明,植物的应激反应和适应是一个复杂的过程。针对基因组序列、转录本、蛋白质组织和相互作用、胁迫特异性和细胞转录组文库以及突变筛选引起的植物非生物胁迫反应的多基因性,可以作为综合方法的第一步。因此,在这篇综述中,我们重点关注了番茄在非生物胁迫下的基因组、蛋白质组和代谢组学。