Department of Preclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, Champ Fleurs, Trinidad and Tobago.
J Med Food. 2010 Oct;13(5):1141-6. doi: 10.1089/jmf.2009.0214.
The objective of our study is to investigate the wound healing activity of the bark extract of Carapa guianensis in rats using three different wound models. The animals were randomly divided into two groups of six each in all the models. Test group animals were treated topically with the bark extract (200 mg/kg of body weight), and the controls were treated with petroleum jelly in the excision wound model. In the incision and dead space wound models the test group animals were treated with the extract of C. guianensis (200 mg/kg/day) orally by mixing in drinking water, and the control group animals were maintained with plain drinking water. Healing was assessed by the rate of wound contraction, period of epithelialization, skin breaking strength, granulation tissue weight, and hydoxyproline content. Antimicrobial activities of the extract against the microorganisms were also assessed. On day 15 extract-treated animals exhibited 99% reduction in the wound area compared to controls (93%). The extract-treated wounds were found to epithelialize faster than controls (P < .02). The skin breaking strength was significantly higher in extract-treated animals compared to controls (P < .001). The weight and hydroxyproline content of granulation tissue were significantly increased compared to controls. These observations support the use of C. guianensis is in the management of wound healing.
本研究的目的是通过三种不同的创伤模型，研究圭亚那卡帕拉树皮提取物对大鼠的创伤愈合活性。所有模型中，动物均随机分为两组，每组 6 只。实验组动物局部给予树皮提取物（200mg/kg 体重），对照组在切除伤模型中给予凡士林治疗。在切口和死腔伤模型中，实验组动物通过在饮用水中混合，口服给予 C. guianensis 提取物（200mg/kg/天）进行治疗，对照组动物维持饮用普通水。通过伤口收缩率、上皮化时间、皮肤破裂强度、肉芽组织重量和羟脯氨酸含量来评估愈合情况。还评估了提取物对微生物的抗菌活性。在第 15 天，与对照组（93%）相比，提取物处理的动物的伤口面积减少了 99%（99%）。与对照组相比，实验组的伤口更快地上皮化（P<.02）。与对照组相比，实验组的皮肤破裂强度显著更高（P<.001）。与对照组相比，肉芽组织的重量和羟脯氨酸含量显著增加。这些观察结果支持使用 C. guianensis 来管理创伤愈合。
