Department of Odontology, Federal University of Pará, Belém, Belém, Pará, Brazil.
, Belém, Brazil.
Clin Oral Investig. 2018 Jun;22(5):2069-2079. doi: 10.1007/s00784-017-2300-2. Epub 2017 Dec 18.
The aim of this study was to investigate the healing activity of andiroba (Carapa guianensis Aubl.) against oral mucositis (OM) induced by 5-fluorouracil in golden Syrian hamsters.
A total of 122 animals were randomized and divided into six groups: andiroba oil 100%, andiroba oil 10%, andiroba oil 10% refined, no treatment group, all n = 28; and negative control (NC) and cyclophosphamide (CPA) groups, both n = 5. OM was induced by intraperitoneal administration of 60 mg/kg 5-FU on days 0, 5 and 10 followed by mechanical trauma on the oral mucosa on days 1 and 2. From day 1 to day 15, the animals of the andiroba group were treated three times a day. On days 4, 8, 12 and 15, the mucosa was photographed and removed for clinical and histopathological analysis. The bone marrow of the femur was removed and the micronucleus test was performed to evaluate the cytotoxicity and genotoxicity. The data were subjected to analysis of variance, followed by the Tukey and Bonferroni test.
Treatment with 100% andiroba oil reduced the degree of OM compared to that reported in the other groups (p < 0.05). Andiroba oil at both concentrations was not cytotoxic, but treatment with 100% andiroba oil showed a genotoxic potential (p < 0.001).
Frequent administration of andiroba oil accelerated the healing process in an experimental model of 5-fluorouracil-induced OM. However, the genotoxicity of andiroba in other cell systems and under other conditions are being tested.
The use of andiroba in topical form may be associated with reduced intensity of OM. Seek therapeutic alternatives to minimize the pain and suffering that these side effects cause cancer patients is an important scientific step.
本研究旨在探讨巴西栗（Carapa guianensis Aubl.）油对 5-氟尿嘧啶诱导的金黄地鼠口腔黏膜炎（OM）的愈合活性。
将 122 只动物随机分为 6 组：巴西栗油 100%、巴西栗油 10%、巴西栗油 10%精炼、未治疗组，每组 n = 28；无处理组（NC）和环磷酰胺（CPA）组，每组 n = 5。第 0、5 和 10 天通过腹腔注射 60mg/kg 5-FU 诱导 OM，第 1 和 2 天通过机械创伤损伤口腔黏膜。从第 1 天到第 15 天，巴西栗组的动物每天治疗 3 次。在第 4、8、12 和 15 天，对黏膜进行拍照并取出进行临床和组织病理学分析。从股骨中取出骨髓，进行微核试验以评估细胞毒性和遗传毒性。对数据进行方差分析，然后进行 Tukey 和 Bonferroni 检验。
与其他组相比，100%巴西栗油治疗降低了 OM 的严重程度（p < 0.05）。两种浓度的巴西栗油均无细胞毒性，但 100%巴西栗油治疗显示出遗传毒性潜力（p < 0.001）。
频繁给予巴西栗油加速了 5-氟尿嘧啶诱导的 OM 实验模型的愈合过程。然而，正在测试巴西栗在其他细胞系统和其他条件下的遗传毒性。
局部使用巴西栗油可能与 OM 强度降低有关。寻求治疗替代方案以最大程度地减少这些副作用给癌症患者带来的疼痛和痛苦是一个重要的科学步骤。
