Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Biomaterials. 2010 Dec;31(34):8989-95. doi: 10.1016/j.biomaterials.2010.08.029. Epub 2010 Sep 15.
We developed a high throughput micro-arrayed polymer system for the study of polymer surfaces for islet cell culture. A micro-arrayed library with 496 different polymers was synthesized and used to examine attachment and insulin expression of islet cells. While most polymers were not supportive, several related polymers were identified as suitable ("hit's"). The "hit" arrays composed of "hit" polymers with 36 replicates were fabricated to confirm their capacities to support the attachment of islet cells, and these capacities were further validated in large surfaces. Notably, the attachment of islet cells on these synthetic polymeric films has been found to be as supportive as 804G supernatant coated tissue culture polystyrene dishes, one of the most extensively used substrates for the islet cell attachment. Interestingly, the polymeric surfaces optimal for a different cell type, hES derived cells, were distinct, highlighting the utility of these approaches for identifying cell type specific surfaces.
我们开发了一种高通量的微阵列聚合物系统，用于研究用于胰岛细胞培养的聚合物表面。合成了具有 496 种不同聚合物的微阵列文库，并用于研究胰岛细胞的附着和胰岛素表达。虽然大多数聚合物不支持，但鉴定出几种相关聚合物是合适的（“命中”）。由具有 36 个重复的“命中”聚合物组成的“命中”阵列被制造出来以确认它们支持胰岛细胞附着的能力，并且这些能力在更大的表面上得到了进一步验证。值得注意的是，已经发现胰岛细胞在这些合成聚合物薄膜上的附着与最广泛用于胰岛细胞附着的基质之一 804G 上清液包被的组织培养聚苯乙烯（tissue culture polystyrene，TCPS）具有相同的支持作用。有趣的是，对于不同细胞类型，胚胎干细胞（human embryonic stem cells，hES）衍生的细胞，最佳的聚合物表面是不同的，这突出了这些方法在鉴定细胞类型特异性表面方面的实用性。
