School of Chemistry, The University of Edinburgh, Kings Buildings, West Mains Road, EH9 3JJ, UK.
Biomaterials. 2010 Mar;31(8):2216-28. doi: 10.1016/j.biomaterials.2009.11.101. Epub 2010 Jan 13.
A combination of high-throughput material formulation and microarray techniques were synergistically applied for the efficient analysis of the biological functionality of 135 binary polymer blends. This allowed the identification of cell-compatible biopolymers permissive for human skeletal stem cell growth in both in vitro and in vivo applications. The blended polymeric materials were developed from commercially available, inexpensive and well characterised biodegradable polymers, which on their own lacked both the structural requirements of a scaffold material and, critically, the ability to facilitate cell growth. Blends identified here proved excellent templates for cell attachment, and in addition, a number of blends displayed remarkable bone-like architecture and facilitated bone regeneration by providing 3D biomimetic scaffolds for skeletal cell growth and osteogenic differentiation. This study demonstrates a unique strategy to generate and identify innovative materials with widespread application in cell biology as well as offering a new reparative platform strategy applicable to skeletal tissues.
高通量材料配方和微阵列技术的结合被协同应用于 135 种二元聚合物共混物的生物功能的有效分析。这使得能够鉴定出在体外和体内应用中允许人骨肉干细胞生长的细胞相容的生物聚合物。混合的聚合物材料是由商业上可获得的、廉价的和特征良好的可生物降解聚合物开发的，这些聚合物本身既缺乏支架材料的结构要求，也缺乏促进细胞生长的能力。这里鉴定的共混物被证明是细胞附着的极好模板，此外，一些共混物显示出显著的类骨结构，并通过为骨骼细胞生长和成骨分化提供 3D 仿生支架来促进骨再生。这项研究展示了一种独特的策略，用于生成和识别具有广泛应用于细胞生物学的创新材料，并提供了一种适用于骨骼组织的新的修复平台策略。
