INSERM-Université Paris Diderot UMR 738, Paris, France.
Comput Methods Programs Biomed. 2011 Oct;104(1):10-8. doi: 10.1016/j.cmpb.2010.08.003. Epub 2010 Sep 9.
Malaria remains one of the leading causes of morbidity and mortality worldwide which is partly due to the emergence of the parasite resistance to antimalarial drugs. In vitro testing of drugs allows an early detection of resistance. The common parameter used for the monitoring of resistance is the concentration that inhibits 50% of the parasite's activity (IC(50)). Various methods of calculation are already used but none of them consider new available tools nor display the precision of IC(50) estimation. We proposed an approach based on the inhibitory sigmoid E(max) model, which is often used in pharmacology, with estimation of IC(50) through nonlinear regression using a standard function of the R software. To facilitate the usage of that tool we have developed an online version available on the website ICEstimator (ICEstimator website http://www.antimalarial-icestimator.net/, 2009) [1]. This website is used by various teams in the world and the user can do the analysis without knowing R using the GUI. This article describes version 2.1 of this website and shows illustration on five different real examples.
疟疾仍然是全球发病率和死亡率的主要原因之一，部分原因是寄生虫对抗疟药物产生了耐药性。药物的体外测试可以早期发现耐药性。监测耐药性的常用参数是抑制寄生虫 50%活性的浓度（IC50）。已经使用了各种计算方法，但没有一种方法考虑到新的可用工具，也没有显示出 IC50估计的精度。我们提出了一种基于抑制性 S 型 E(max)模型的方法，该模型常用于药理学，通过使用 R 软件的标准函数进行非线性回归来估计 IC50。为了方便使用该工具，我们开发了一个在线版本，可在 ICEstimator 网站上获得（ICEstimator 网站：http://www.antimalarial-icestimator.net/，2009 年）[1]。该网站被世界各地的不同团队使用，用户可以使用 GUI 而无需了解 R 就可以进行分析。本文介绍了该网站的 2.1 版本，并通过五个不同的真实示例进行了说明。
