Faculty of Chemistry and Technology, Department of Biochemistry, University of Split, Teslina 10/V, Split 21000, Croatia.
Nat Prod Res. 2012;26(18):1703-7. doi: 10.1080/14786419.2011.602639. Epub 2011 Oct 19.
The methanol, ethyl acetate and chloroform extracts of selected Croatian plants were tested for their acetylcholinesterase (AChE) inhibition and antioxidant activity. Assessment of AChE inhibition was carried out using microplate reader at 1 mg mL⁻¹. Antioxidant capacities were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test and ferric reducing/antioxidant power assay (FRAP). Total phenol content (TPC) of extracts were determined using Folin-Ciocalteu colorimetric method. Out of 48 extracts, only methanolic extract of the Salix alba L. cortex exerted modest activity towards AChE, reaching 50.80% inhibition at concentration of 1 mg mL⁻¹. All the other samples tested had activity below 20%. The same extract performed the best antioxidative activity using DPPH and FRAP method, too. In essence, among all extracts used in the screening, methanolic extracts showed the best antioxidative activity as well as highest TPC.
对来自克罗地亚的部分植物的甲醇、乙酸乙酯和氯仿提取物进行了乙酰胆碱酯酶（AChE）抑制和抗氧化活性测试。在 1mg/mL 的浓度下，使用微孔板读取器对 AChE 抑制进行了评估。通过 2,2-二苯基-1-苦基肼（DPPH）自由基清除试验和铁还原/抗氧化能力测定（FRAP）测定了抗氧化能力。使用福林-肖卡（Folin-Ciocalteu）比色法测定了提取物的总酚含量（TPC）。在 48 种提取物中，只有柳皮的甲醇提取物对 AChE 表现出适度的活性，在 1mg/mL 的浓度下达到了 50.80%的抑制率。其他所有测试的样品活性均低于 20%。同一样品在使用 DPPH 和 FRAP 方法时也表现出了最佳的抗氧化活性。实际上，在所筛选的所有提取物中，甲醇提取物的抗氧化活性以及总酚含量最高。
