Anti-inflammatory effect of leaves based on TNF-α, IL-1, IL-6 and antioxidant potential.

OBJECTIVE

In this study, we evaluated anti-inflammatory activity of leaves of () and underlying mechanisms by using models of inflammation.

MATERIAL AND METHODS

Albino Wistar rats of either sex weighing 150 - 200 g were used. Soxhlet ethanol and aqueous extracts of leaves of (EFP and AFP) were prepared. The anti-inflammatory activity was studied using carrageenan-induced paw edema method and cotton pellet granuloma method. Levels of cytokines such as TNF-α, IL-6 and IL-1 and activity of antioxidant enzymes including catalase (CAT) and glutathione peroxidase (GPx) were estimated.

RESULTS

Leaves of demonstrated significant (p<0.001) decrease in paw edema in carrageenan-induced paw edema method. It diminished the serum tumour necrosis factor-α (TNF-α), IL-6 and IL-1 levels and also significantly attenuated the malondialdehyde (MDA) levels. The activity of CAT and GPx was increased in paw tissue. It also demonstrated significant decrease in granuloma formation in cotton pellet-induced granuloma method.

CONCLUSION

Leaves of possess anti-inflammatory activity as they inhibit various cytokines and have antioxidant effects and free radical scavenging activity.