Institute of Forensic Genetics, 48161, Münster, Germany.
Int J Legal Med. 2010 Nov;124(6):617-22. doi: 10.1007/s00414-010-0506-9. Epub 2010 Sep 10.
Horse mtDNA profiling can be useful in forensic work investigating degraded samples, hair shafts or highly dilute samples. Degraded DNA often does not allow sequencing of fragments longer than 200 nucleotides. In this study we therefore search for the most discriminatory sections within the hypervariable horse mtDNA control region. Among a random sample of 39 horses, 32 different sequences were identified in a stretch of 921 nucleotides. The sequences were assigned to the published mtDNA types A-G, and to a newly labelled minor type H. The random match probability within the analysed samples is 3.61%, and the average pairwise sequence difference is 15 nucleotides. In a "sliding window" analysis of 200-nucleotide sections of the mtDNA control region, we find that the known repetitive central motif divides the mtDNA control region into a highly diverse segment and a markedly less discriminatory segment.
马的 mtDNA 分析在法医学研究中可以用于检测降解样本、毛发或高度稀释的样本。降解的 DNA 通常不允许对超过 200 个核苷酸的片段进行测序。因此，在本研究中，我们在高度可变的马 mtDNA 控制区中寻找最具区分度的部分。在对 39 匹马的随机样本进行分析时，在 921 个核苷酸的片段中鉴定出了 32 种不同的序列。这些序列被分配到已发表的 mtDNA 类型 A-G 中，并被一个新标记的次要类型 H 所标记。在分析样本中的随机匹配概率为 3.61%，平均成对序列差异为 15 个核苷酸。在对 mtDNA 控制区 200 个核苷酸片段的“滑动窗口”分析中，我们发现已知的重复中央基序将 mtDNA 控制区分为高度多样化的片段和明显区分度较低的片段。
