Department of General and Analytical Chemistry, Faculty of Pharmacy, Istanbul University, 34116, Istanbul, Turkey.
Phytother Res. 2011 Apr;25(4):522-9. doi: 10.1002/ptr.3299. Epub 2010 Sep 9.
The aim of this study was to investigate the phytochemical, antioxidant and anticholinesterase activities of Iris suaveolens. After determining total phenolic and flavonoid contents of the petroleum ether, chloroform and methanol extracts prepared from the rhizomes, the antioxidant capacity of the extracts was established using β-carotene-linoleic acid and CUPRAC methods. The chloroform extract which was rich in phenolic content exhibited the highest inhibition of lipid peroxidation in the β-carotene-linoleic acid system, and the best cupric reducing antioxidant capacity among the tested extracts. The petroleum ether extract indicated moderate anticholinesterase activity while the chloroform extract revealed significant butyrylcholinesterase inhibitory activity (75.03 ± 1.29%). Spectroscopic methods were used for the structural elucidation of the compounds (1-13) isolated from the petroleum ether and chloroform extracts. Coniferaldehyde (6), having the highest antioxidant activity in the β-carotene-linoleic acid assay at 25 and 50 µg/mL, demonstrated also the best effect in the CUPRAC method among the tested compounds (1-12). 3-Hydroxyirisquinone (10) showed the best anticholinesterase activity among the tested compounds (1-4, 6-12), and coniferaldehyde exhibited almost the same butyrylcholinesterase inhibitory activity (82.60 ± 2.33%) as galantamine (86.26 ± 0.66%).
本研究旨在探讨鸢尾的植物化学成分、抗氧化和乙酰胆碱酯酶抑制活性。在确定根茎的石油醚、氯仿和甲醇提取物中的总酚和类黄酮含量后，使用β-胡萝卜素-亚油酸和 CUPRAC 方法确定提取物的抗氧化能力。富含酚类化合物的氯仿提取物在β-胡萝卜素-亚油酸体系中表现出最高的抑制脂质过氧化作用，并且在测试的提取物中具有最佳的铜还原抗氧化能力。石油醚提取物表现出中等的乙酰胆碱酯酶抑制活性，而氯仿提取物则表现出显著的丁酰胆碱酯酶抑制活性（75.03 ± 1.29%）。光谱学方法用于鉴定从石油醚和氯仿提取物中分离得到的化合物（1-13）的结构。在 25 和 50μg/mL 的β-胡萝卜素-亚油酸测定中，具有最高抗氧化活性的松柏醛（6）在测试的化合物（1-12）中也表现出最佳的 CUPRAC 方法效果。在测试的化合物（1-4、6-12）中，3-羟基鸢尾醌（10）表现出最佳的乙酰胆碱酯酶抑制活性，而松柏醛则表现出几乎与加兰他敏（82.60 ± 2.33%）相同的丁酰胆碱酯酶抑制活性（86.26 ± 0.66%）。
