Agner T, Fullerton A, Broby-Johansen U, Batsberg W
Department of Dermatology, Rigshospitalet, Copenhagen, Denmark.
Skin Pharmacol. 1990;3(4):213-7. doi: 10.1159/000210873.
To clarify the sources of variation in irritant patch testing, variability in delivery of the test substance from the patch test system was studied. An in vitro model was used to study the penetration of sodium lauryl sulphate (SLS) from a patch test system into the skin. Different formulations of SLS applied to the skin for 24 h were studied (aqueous solution and gels), but irrespective of the vehicle used permeation of SLS into the recipient phase was poor. Results were compared to in vivo patch testing in 12 subjects. Approximately 70% of the applied SLS in aqueous solution was released from the patch test system. Release from gels was poorer. High concordance between the in vivo and the in vitro model was found. No correlation was found between the amount of SLS left in the filter disc and the strength of the clinical reaction in vivo.
为了阐明刺激性斑贴试验中变异的来源，对斑贴试验系统中测试物质递送的变异性进行了研究。使用体外模型研究月桂醇硫酸酯钠（SLS）从斑贴试验系统渗透到皮肤中的情况。研究了将不同配方的SLS应用于皮肤24小时的情况（水溶液和凝胶），但无论使用何种载体，SLS向接受相的渗透都很差。将结果与12名受试者的体内斑贴试验进行了比较。水溶液中约70%的应用SLS从斑贴试验系统中释放出来。从凝胶中的释放较差。发现体内和体外模型之间具有高度一致性。在滤盘中剩余的SLS量与体内临床反应强度之间未发现相关性。
