Frankild S, Andersen K E, Nielsen G D
Department of Dermato-venereology, Odense University Hospital, Denmark.
Contact Dermatitis. 1995 Jun;32(6):338-45. doi: 10.1111/j.1600-0536.1995.tb00622.x.
The dose- and time-related effect of sodium lauryl sulfate (SLS) on in vitro percutaneous penetration was studied using 3 radiolabeled tracer compounds with different physicochemical properties: tritiated water, hydrocortisone and nickel. Human cadaver abdominal skin from caucasian women was used as membrane in static in vitro penetration cells. Simultaneous application of SLS together with 1 of the tracer compounds showed, after 48 h, a significant dose-effect relationship between SLS concentration (0.25%, 2% and 10%) and penetration of tritiated water or nickel (p < 0.001, Spearman), whereas SLS had no significant effect on penetration of hydrocortisone. When 4% SLS was applied as pretreatment, a significant time-effect relationship, after 48 h, was found between pretreatment time (0.5, 2 and 8 h) and penetration of tritiated water. A similar relationship was not found for penetration of nickel or hydrocortisone. Pretreatment of the skin with SLS for 2 h using 3 concentrations (0.25%, 4% and 10%) showed, after 48 h, a significant dose-effect relationship between SLS treatment and penetration of tritiated water or nickel (p < 0.001, Spearman). Pretreatment had no effect on penetration of hydrocortisone. Pretreatment simulates a cleaning-washing situation. The present in vitro skin penetration model, using human cadaver skin, described the dose-effect and time-effect relationships for SLS on the penetration profiles of 3 different compounds. The model may be extended to other compounds with suspected irritant/damaging effect on the skin barrier. It should be kept in mind that the model uses a dead skin membrane without the barrier repair mechanisms of live skin.
使用三种具有不同物理化学性质的放射性标记示踪化合物（氚水、氢化可的松和镍）研究了月桂醇硫酸酯钠（SLS）在体外经皮渗透方面的剂量和时间相关效应。来自白种女性的人类尸体腹部皮肤被用作静态体外渗透细胞中的膜。将SLS与一种示踪化合物同时应用48小时后，SLS浓度（0.25%、2%和10%）与氚水或镍的渗透之间存在显著的剂量效应关系（p < 0.001，Spearman），而SLS对氢化可的松的渗透没有显著影响。当应用4%的SLS进行预处理时，48小时后发现预处理时间（0.5、2和8小时）与氚水的渗透之间存在显著的时间效应关系。对于镍或氢化可的松的渗透未发现类似关系。使用三种浓度（0.25%、4%和10%）的SLS对皮肤进行2小时预处理，48小时后，SLS处理与氚水或镍的渗透之间存在显著的剂量效应关系（p < 0.001，Spearman）。预处理对氢化可的松的渗透没有影响。预处理模拟了清洁洗涤的情况。目前使用人类尸体皮肤的体外皮肤渗透模型描述了SLS对三种不同化合物渗透曲线的剂量效应和时间效应关系。该模型可扩展到对皮肤屏障有疑似刺激/损伤作用的其他化合物。应记住，该模型使用的是死皮肤膜，没有活皮肤的屏障修复机制。
