School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India.
Mol Immunol. 2010 Nov-Dec;48(1-3):264-71. doi: 10.1016/j.molimm.2010.08.001. Epub 2010 Sep 15.
Heat shock proteins (Hsps) of the HSP60 and HSP70 family are highly conserved and essential to all living organisms. Hsps are immunodominant in numerous microbial infections and have been investigated for their vaccine potential. We investigated the immunogenicity and protective efficacy of GroEL and DnaK of B. anthracis in murine model. Both Hsps were found to be highly immunogenic with mixed antibody response (both IgG1 and IgG2a), indicating stimulation of both humoral and cell-mediated immunity. Cytokine profile also confirmed robust T-cell response with increase in lymphocyte proliferation. Immunization with GroEL conferred 100% protection to mice against B. anthracis infection whereas DnaK couldn't provide protection.
热休克蛋白（Hsps）属于 HSP60 和 HSP70 家族，高度保守，是所有生物的必需物质。Hsps 在许多微生物感染中具有免疫优势，并已被研究用于疫苗潜力。我们在小鼠模型中研究了炭疽杆菌的 GroEL 和 DnaK 的免疫原性和保护效力。结果发现这两种 Hsp 都具有高度的免疫原性，产生混合抗体反应（IgG1 和 IgG2a），表明体液和细胞介导免疫均受到刺激。细胞因子谱也证实了强烈的 T 细胞反应，淋巴细胞增殖增加。GroEL 免疫可使小鼠对炭疽杆菌感染 100%产生保护作用，而 DnaK 则不能提供保护。
