Discipline of Oral and Maxillofacial Surgery, Department of Surgery and Integrated Clinics, Araçatuba Dental School, São Paulo State University, Araçatuba, SP, Brazil.
J Appl Oral Sci. 2010 Jul-Aug;18(4):346-53. doi: 10.1590/s1678-77572010000400005.
Although the search for the ideal bone substitute has been the focus of a large number of studies, autogenous bone is still the gold standard for the filling of defects caused by pathologies and traumas, and mainly, for alveolar ridge reconstruction, allowing the titanium implants installation.
The aim of this study was to evaluate the dynamics of autogenous bone graft incorporation process to surgically created defects in rat calvaria, using epifluorescence microscopy.
Five adult male rats weighing 200-300 g were used. The animals received two 5-mm-diameter bone defects bilaterally in each parietal bone with a trephine bur under general anesthesia. Two groups of defects were formed: a control group (n=5), in which the defects were filled with blood clot, and a graft group (n=5), in which the defects were filled with autogenous bone block, removed from the contralateral defect. The fluorochromes calcein and alizarin were applied at the 7th and 30th postoperative days, respectively. The animals were killed at 35 days.
The mineralization process was more intense in the graft group (32.09%) and occurred mainly between 7 and 30 days, the period labeled by calcein (24.66%).
The fluorochromes showed to be appropriate to label mineralization areas. The interfacial areas between fluorochrome labels are important sources of information about the bone regeneration dynamics.
尽管人们一直在寻找理想的骨替代物，但自体骨仍然是填充因疾病和创伤引起的缺陷的金标准，主要用于牙槽嵴重建，以允许钛植入物的安装。
本研究旨在使用荧光显微镜评估自体骨移植物在大鼠颅骨手术性缺损中的吸收过程的动力学。
使用 5 只成年雄性大鼠，体重 200-300g。在全身麻醉下，用环钻在每块顶骨上双侧形成两个 5mm 直径的骨缺损。形成两组缺陷：对照组（n=5），其中缺陷用血凝块填充；移植物组（n=5），其中缺陷用取自对侧缺陷的自体骨块填充。在术后第 7 天和第 30 天分别应用钙黄绿素和茜素红两种荧光染料。在第 35 天处死动物。
移植物组的矿化过程更强烈（32.09%），主要发生在第 7 天至第 30 天之间，即钙黄绿素标记的时期（24.66%）。
荧光染料适用于标记矿化区域。荧光染料标记的界面区域是骨再生动力学的重要信息来源。
