Shimomura Y, Shimizu H, Takahashi M, Uehara Y, Negishi M, Kobayashi I, Kobayashi S
Department of Medicine, Gunma University School of Medicine, Maebashi, Japan.
Exp Clin Endocrinol. 1990 Dec;96(3):296-300. doi: 10.1055/s-0029-1211022.
We investigated whether the difference of insulin effect existed or not when insulin was administered at different times; just before the light phase (am 6:00) (L-NPH-DM) or just before the dark phase (pm 6:00) (D-NPH-DM) in streptozotocin (60 mg/kg, ip)-induced diabetic rats. Following results were obtained 7 days after STZ administration. Body weight: A significant increase compared to (D-NPH-DM) group was observed in (L-NPH-DM) group. Food intake: Food intake gradually increased after administration of STZ. A significant decrease compared to (saline-DM) group was observed only in the (L-NPH-DM) group. Total lipid content in the liver: A significant increase compared to (saline-control) group was seen in both the (D-saline-DM) and (L-saline-DM) group. In addition, a significant decrease compared to (D-NPH-DM) was observed in the (L-NPH-DM) group. Changes in blood glucose: Different patterns in decrease of blood glucose was observed between (D-NPH-DM) and (L-NPH-DM) groups. In conclusion, the present study demonstrates for the first time that there are differences of insulin effects on body weight, food intake, total lipid content in the liver and blood glucose level with insulin administration time.
我们研究了在链脲佐菌素（60毫克/千克，腹腔注射）诱导的糖尿病大鼠中，在不同时间（光照期开始前6:00上午（L-NPH-DM）或黑暗期开始前6:00下午（D-NPH-DM））注射胰岛素时，胰岛素效应是否存在差异。在给予链脲佐菌素7天后获得了以下结果。体重：（L-NPH-DM）组与（D-NPH-DM）组相比体重显著增加。食物摄入量：给予链脲佐菌素后食物摄入量逐渐增加。仅在（L-NPH-DM）组观察到与（生理盐水-DM）组相比有显著下降。肝脏中的总脂质含量：（D-生理盐水-DM）组和（L-生理盐水-DM）组与（生理盐水-对照）组相比均显著增加。此外，（L-NPH-DM）组与（D-NPH-DM）组相比有显著下降。血糖变化：（D-NPH-DM）组和（L-NPH-DM）组之间观察到血糖下降的不同模式。总之，本研究首次证明，胰岛素给药时间不同，胰岛素对体重、食物摄入量、肝脏总脂质含量和血糖水平的影响存在差异。
