Cátedra de Microbiología General, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
Mycopathologia. 2011 May;171(5):361-71. doi: 10.1007/s11046-010-9362-x. Epub 2010 Sep 12.
Leaf blight and purple seed, caused by the fungal pathogen Cercospora kikuchii (Matsumoto & Tomoyasu) M. W. Gardner are very important diseases of soybean (Glycine max L. Merr.) in Argentina. The aims of this work were: (a) to confirm and to assess the genetic variability among C. kikuchii isolates collected from different soybean growing areas in Santa Fe province using inter simple sequence repeats (ISSR) markers and sequence information from the internal transcribed spacer (ITS) region of rDNA and (b) to analyze the cercosporin production of the regional C. kikuchi isolates in order to assess whether there was any relationship between the molecular profiles and the toxin production. Isolates from different regions in Santa Fe province were studied. The sequence of the ITS regions showed high similarity (99-100%) to the GenBank sequences of C. kikuchii BRCK179 (accession number AY633838). The ISSR markers clustered all the isolates into many groups and cercosporin content was highly variable among isolates. No relationship was observed between ITS region, ISSR groups and origin or cercosporin content. The high degree of genetic variability and cercosporin production among isolates compared in this study characterizes a diverse population of C. kikuchii in the region.
叶片枯萎病和紫种皮病是由真菌病原菌 Cercospora kikuchii（Matsumoto & Tomoyasu）M. W. Gardner 引起的，是阿根廷大豆（Glycine max L. Merr.）的重要病害。本研究的目的是：（a）利用 ISSR 标记和 rDNA 内转录间隔区（ITS）序列信息，确认并评估来自圣达菲省不同大豆种植区的 C. kikuchii 分离株的遗传变异性；（b）分析该地区 C. kikuchii 分离株的尾孢菌素产量，以评估分子图谱与毒素产生之间是否存在任何关系。对来自圣达菲省不同地区的分离株进行了研究。ITS 区序列与 C. kikuchii BRCK179（登录号 AY633838）的 GenBank 序列高度相似（99-100%）。ISSR 标记将所有分离株聚类为许多组，且尾孢菌素含量在分离株间高度可变。ITS 区、ISSR 组与起源或尾孢菌素含量之间未观察到相关性。本研究中比较的分离株之间存在高度的遗传变异性和尾孢菌素产生，表明该地区 C. kikuchii 存在多样化的种群。
