麦角甾醇生物合成抑制剂对烟草细胞膜组成和结构的影响。
Changes in tobacco cell membrane composition and structure caused by cercosporin.
机构信息
Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48824.
出版信息
Plant Physiol. 1983 Apr;71(4):763-6. doi: 10.1104/pp.71.4.763.
Abstract
Cercosporin, a toxin produced by Cercospora species, rapidly kills plant cells in the light. Previous work has shown that cercosporin treatment causes products of lipid peroxidation to be released. We have found that the unsaturated acyl chains of lipids in tobacco (Nicotiana tabacum) cell membranes are destroyed when cells are treated with cercosporin. Concomitant with this change in composition is a change in structure of the membranes as detected by two different fatty acid spin labels, 2-(3-carboxypropyl)-4,4-dimethyl-2-tridecyl-3-oxazolidinyloxyl (denoted I[12,3]) and 2-(14-carboxytetradecyl)-2-ethyl-4,4-dimethyl-3-oxazolidinyloxyl (denoted I[1,14]). Cercosporin causes the membranes to become more rigid at all temperatures tested and increases the membrane phase transformation temperature from 12.7 degrees C to 20.8 degrees C.
摘要
尾孢菌素，一种由尾孢菌属产生的毒素，在光照下能迅速杀死植物细胞。先前的研究表明，尾孢菌素处理会导致脂质过氧化产物的释放。我们发现，当用尾孢菌素处理细胞时，烟草（Nicotiana tabacum）细胞膜中的不饱和酰基链会被破坏。与这种成分变化相伴的是膜结构的变化，这可以通过两种不同的脂肪酸旋转标记物 2-(3-羧丙基)-4,4-二甲基-2-十三烷基-3-恶唑啉氧基（表示为 I[12,3]）和 2-(14-羧基十四烷基)-2-乙基-4,4-二甲基-3-恶唑啉氧基（表示为 I[1,14]）检测到。尾孢菌素使膜在所有测试温度下变得更加刚性，并将膜相变温度从 12.7°C 提高到 20.8°C。
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