Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, Republic of Korea.
Anal Chim Acta. 2010 Sep 16;677(2):162-8. doi: 10.1016/j.aca.2010.08.004. Epub 2010 Aug 13.
Xylose and glucose, as the main hydrolyzed products of plant cell wall, were separated by silica-confined ionic liquid (IL) stationary phases. Five different stationary phases were synthesized and characterized. Instead of using the traditional NH(2) column, the imidazolium stationary phases exhibit excellent retention to the xylose and glucose. The retention factor and resolution of the monosaccharides decreased with decreasing acetonitrile concentration. In addition, the effects of the IL cations and anions on the retention of xylose and glucose were studied and the adsorption behavior of these two monosaccharides on the stationary phases was investigated. Then the mobile phase and temperature were optimized to improve the performance for the separation of xylose and glucose.
木糖和葡萄糖是植物细胞壁的主要水解产物，它们被硅胶限域离子液体（IL）固定相分离。合成并表征了五种不同的固定相。与传统的 NH(2)柱不同，离子液体固定相对木糖和葡萄糖表现出极好的保留。随着乙腈浓度的降低，单糖的保留因子和分辨率降低。此外，还研究了 IL 阳离子和阴离子对木糖和葡萄糖保留的影响，并研究了这两种单糖在固定相上的吸附行为。然后优化了流动相和温度，以提高分离木糖和葡萄糖的性能。
