Laboratório de Fitoquímica, Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais - UFMG, Belo Horizonte MG, Brazil.
Lett Appl Microbiol. 2010 Oct;51(4):469-76. doi: 10.1111/j.1472-765X.2010.02924.x.
To evaluate the antiviral activity of Bignoniaceae species occurring in the state of Minas Gerais, Brazil.
Ethanol extracts of different anatomical parts of bignoniaceous plant species have been evaluated in vitro against human herpesvirus type 1 (HSV-1), vaccinia virus (VACV) and murine encephalomyocarditis virus (EMCV) by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. A total of 34 extracts from 18 plant species selected according to ethnopharmacological and taxonomic criteria were screened. Fifteen of the 34 extracts (44.1%) have disclosed antiviral activity against one or more of the viruses assayed with EC(50) values in the range of 23.2 ± 2.5-422.7 ± 10.9 μg ml(-1).
Twelve of the 34 extracts (35.3%) might be considered promising sources of antiviral natural products, as they have shown EC50 ≤ 100 μg ml(-1). The present screening discloses the high potential of the Bignoniaceae family as source of antiviral agents.
Active extracts were identified and deserve bioguided studies for the isolation of antiviral compounds and studies on mechanism of action.
评估巴西米纳斯吉拉斯州的紫葳科植物的抗病毒活性。
通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴化物测定法，评估了不同紫葳科植物解剖部位的乙醇提取物对人疱疹病毒 1（HSV-1）、牛痘病毒（VACV）和鼠脑心肌炎病毒（EMCV）的体外抗病毒活性。根据民族药理学和分类学标准选择了 18 种植物的 34 种提取物进行筛选。在测定的病毒中，34 种提取物中有 15 种（44.1%）对一种或多种病毒表现出抗病毒活性，EC（50）值范围为 23.2 ± 2.5-422.7 ± 10.9 μg ml（-1）。
在测定的 34 种提取物中，有 12 种（35.3%）可能被认为是具有抗病毒天然产物潜力的来源，因为它们的 EC50 ≤ 100 μg ml（-1）。本研究揭示了紫葳科植物作为抗病毒药物来源的巨大潜力。
已确定活性提取物值得进行生物导向研究，以分离抗病毒化合物并研究作用机制。
