Faculty of Medicine, Department of Medical Biology, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey.
Mol Biol Rep. 2011 Mar;38(3):1469-74. doi: 10.1007/s11033-010-0253-5. Epub 2010 Sep 16.
We aimed to investigate the association of polymorphism frequencies of MMP-2 C1306T and MMP-2 plasma enzyme activity in lung cancer patients. In this study 300 genomic DNA (200 lung cancer patients + 100 no lung cancer) were analyzed. Polymorphisms were determined by using polymerase chain reaction-restriction fragment length polymorphism (RFLP) and electrophoresis. Plasma MMP-2 enzyme activity levels were measured by using ELISA. Sex, asbestos expose and smoking might be risk factors for lung cancer. The frequencies of C1306T genotypes in controls CC 65%, CT 32%, TT 3% and in patients CC 61%, CT 37%, TT2 % were found. It was determined that CC genotype frequency increase significantly in patients and controls. Plasma MMP-2 enzyme activity levels were increased in lung cancer patients according to controls. Finally, we claimed that determining of MMP-2 enzyme level can be used as a marker in lung cancer.
我们旨在研究 MMP-2 C1306T 多态性频率与肺癌患者血浆酶活性的关系。在这项研究中，分析了 300 个基因组 DNA（200 例肺癌患者+100 例非肺癌患者）。多态性通过聚合酶链反应-限制性片段长度多态性（RFLP）和电泳来确定。通过 ELISA 测量血浆 MMP-2 酶活性水平。性别、石棉暴露和吸烟可能是肺癌的危险因素。对照组 CC 基因型频率为 65%，CT 基因型频率为 32%，TT 基因型频率为 3%，而患者组 CC 基因型频率为 61%，CT 基因型频率为 37%，TT 基因型频率为 2%。结果表明，患者和对照组中 CC 基因型频率明显增加。与对照组相比，肺癌患者的血浆 MMP-2 酶活性水平升高。最后，我们认为 MMP-2 酶水平的测定可以作为肺癌的标志物。
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