Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Biochemistry. 2010 Oct 19;49(41):8815-7. doi: 10.1021/bi1012854.
The biosynthesis of many natural products of clinical interest involves large, multidomain enzymes called nonribosomal peptide synthetases (NRPSs). In bacteria, many of the gene clusters coding for NRPSs also code for a member of the MbtH-like protein superfamily, which are small proteins of unknown function. Using MbtH-like proteins from three separate NRPS systems, we show that these proteins copurify with the NRPSs and influence amino acid activation. As a consequence, MbtH-like proteins are integral components of NRPSs.
许多具有临床应用价值的天然产物的生物合成涉及被称为非核糖体肽合成酶（NRPSs）的大型多结构域酶。在细菌中，编码 NRPS 的许多基因簇也编码 MbtH 样蛋白超家族的成员，该超家族是具有未知功能的小蛋白。我们使用来自三个独立 NRPS 系统的 MbtH 样蛋白表明，这些蛋白与 NRPS 共同纯化并影响氨基酸的激活。因此，MbtH 样蛋白是 NRPS 的组成部分。
