Institute of Medical Microbiology, University Hospital, Erlanger Allee 101, Jena, Germany.
Diagn Microbiol Infect Dis. 2010 Oct;68(2):110-6. doi: 10.1016/j.diagmicrobio.2010.06.001.
A commercially available enzyme-linked immunosorbent assay (ELISA) (Virion/Serion [Wuerzburg, Germany]), an indirect fluorescent antibody test (IFAT) (BIOS/Focus [Cypress, CA]), and a nested polymerase chain reaction (PCR) were explored for diagnosis of acute Q fever in reference to time of serum collection. Serum samples of 22 patients with acute Q fever collected around the fifth day of illness were included. A sensitivity of 30% by ELISA and 80% by IFAT (P = 0.1) was found for the first 5 days of illness and 92% by ELISA and 83% by IFAT during the sixth and eleventh day. PCR revealed a positive result in 8 cases (36%) with 6 cases deriving from the first 5 days of illness. We conclude that ELISA aids especially in the diagnosis of Q fever after 5 days of illness. The benefit of PCR as an additional tool to ELISA was especially evident in the early days of serum sampling.
研究了一种市售的酶联免疫吸附测定法（ELISA）（Virion/Serion[德国维尔茨堡]）、间接荧光抗体试验（IFAT）（BIOS/Focus[美国加州赛普拉斯]）和巢式聚合酶链反应（PCR），以评估其在血清采集时间方面对急性 Q 热的诊断价值。共纳入了 22 例急性 Q 热患者在发病后第 5 天左右采集的血清样本。ELISA 在发病后第 5 天内的敏感性为 30%，IFAT 为 80%（P=0.1），而在第 6 天和第 11 天，ELISA 和 IFAT 的敏感性分别为 92%和 83%。PCR 显示 8 例（36%）为阳性，其中 6 例源自发病后第 5 天内。我们得出结论，ELISA 尤其有助于在发病后 5 天内诊断 Q 热。在早期进行血清采样时，PCR 作为 ELISA 的附加工具，其优势尤为明显。
