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开发一种聚合酶链反应-限制性片段长度多态性分析方法，用于从临床样本中区分利什曼原虫/利什曼原虫/利什曼原虫，在突尼斯聚焦点的应用。

Development of a polymerase chain reaction-restriction fragment length polymorphism assay for Leishmania major/Leishmania killicki/Leishmania infantum discrimination from clinical samples, application in a Tunisian focus.

机构信息

Laboratory of Parasitology-Mycology (99UR/08-05), Department of Clinical Biology B, Faculty of Pharmacy, Monastir, Tunisia.

出版信息

Diagn Microbiol Infect Dis. 2010 Oct;68(2):152-8. doi: 10.1016/j.diagmicrobio.2010.06.011.

DOI:10.1016/j.diagmicrobio.2010.06.011
PMID:20846587
Abstract

Topoisomerase II gene of Leishmania genus was used to develop a molecular tool for detection and species differentiation of Leishmania from clinical samples. Identification was achieved by a polymerase chain reaction followed by digestion with 2 restriction endonucleases BstU1 and Taq1. Despite the relatively low sensitivity, it is able to differentiate between 3 complexes responsible for cutaneous leishmaniasis.

摘要

使用利什曼属拓扑异构酶 II 基因开发了一种分子工具，用于从临床样本中检测和区分利什曼原虫。通过聚合酶链反应（PCR）鉴定，并使用 2 种限制内切酶 BstU1 和 Taq1 进行消化。尽管灵敏度相对较低，但它能够区分引起皮肤利什曼病的 3 个复合物。

相似文献

[1]
Development of a polymerase chain reaction-restriction fragment length polymorphism assay for Leishmania major/Leishmania killicki/Leishmania infantum discrimination from clinical samples, application in a Tunisian focus.

Diagn Microbiol Infect Dis. 2010-10

[2]
[Contribution of Leishmania identification using polymerase chain reaction--restriction fragment length polymerase for epidemiological studies of cutaneous leishmaniasis in Tunisia].

Pathol Biol (Paris). 2014-2

[3]
[Differentiation among cutaneous Leishmania species upon amplification of a sequence of dipeptidyl peptidase III encoding gene].

Arch Inst Pasteur Tunis. 2008

[4]
Leishmania spp. identification by polymerase chain reaction-restriction fragment length polymorphism analysis and its applications in French Guiana.

Diagn Microbiol Infect Dis. 2009-9-25

[5]
Advantages and limits of real-time PCR assay and PCR-restriction fragment length polymorphism for the identification of cutaneous Leishmania species in Tunisia.

Trans R Soc Trop Med Hyg. 2011-1

[6]
Leishmaniasis in Turkey: molecular characterization of Leishmania from human and canine clinical samples.

Trop Med Int Health. 2009-11

[7]
Usefulness of a PCR-based method in the detection and species identification of Leishmania from clinical samples.

Pathol Biol (Paris). 2012-12

[8]
Use of PCR-restriction fragment length polymorphism analysis to identify the main new world Leishmania species and analyze their taxonomic properties and polymorphism by application of the assay to clinical samples.

J Clin Microbiol. 2006-2

[9]
Heat-shock protein 70 PCR-RFLP: a universal simple tool for Leishmania species discrimination in the New and Old World.

Parasitology. 2010-5-5

[10]
Twenty-four new human cases of cutaneous leishmaniasis due to Leishmania killicki in Metlaoui, southwestern Tunisia: probable role of Phlebotomus sergenti in the transmission.

Acta Trop. 2012-1-24

引用本文的文献

[1]
Visceral Leishmaniasis in Immunocompetent Hosts in Brescia: A Case Series and Analysis of Cytokine Cascade.

Microorganisms. 2024-2-16

[2]
Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods.

Parasit Vectors. 2022-11-5

[3]
Comparison of Leishmania killicki (syn. L. tropica) and Leishmania tropica Population Structure in Maghreb by Microsatellite Typing.

PLoS Negl Trop Dis. 2015-12-8

[4]
Evolutionary history of Leishmania killicki (synonymous Leishmania tropica) and taxonomic implications.

Parasit Vectors. 2015-4-1

[5]
Molecular epidemiology of imported cases of leishmaniasis in Australia from 2008 to 2014.

PLoS One. 2015-3-3

[6]
Species typing in dermal leishmaniasis.

Clin Microbiol Rev. 2015-4

[7]
Cutaneous leishmaniasis caused by Leishmania killicki, Algeria.

Emerg Infect Dis. 2014-3

[8]
First detection of Leishmania infantum (Kinetoplastida: Trypanosomatidae) in Culicoides spp. (Diptera: Ceratopogonidae).

Parasit Vectors. 2014-1-25

[9]
First molecular epidemiological study of cutaneous leishmaniasis in Libya.

PLoS Negl Trop Dis. 2012-6-19

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