[2008 - 2009年卢龙轮状病毒腹泻流行病学研究]

[Study on the epidemiological of rotavirus diarrhea in Lulong in 2008-2009].

作者信息

Li Dan-Di, Yu Qiu-Li, Qi Shun-Xiang, Xie Yun, Zhang Qing, Cui Shu-Xian, Duan Zhao-Jun

机构信息

State Key Laboratory of Molecular Virology and Genetic Engineering, Beijing 100052, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2010 Feb;24(1):2-4.

PMID:20848835

Abstract

OBJECTIVE

To analyze the feature of epidemiological of rotavirus diarrhea in Lulong county, Hebei province.

METHODS

426 stool specimens were collected from inpatant with acute diarrhea from children less than 5 years old. Rotavirus-positive specimens were identified by ELISA kit. G/P typing assays were confirmed with multiplex seminested RT-PCR.

RESULTS

Rotavirus was detected in 202 of 426 (47.42%) specimens. Genotyping of rotavirus showed that G3 was predominant (57.9%), followed by Gmix (16.3%), G9 (14.9% ), G1 (7.9%), G4 (1%), G2 (0.5%), P-genotyping showed that P [8], Pmix, P [4], P [9], type were found in 58.4%, 28.7%, 6.9% and 1% respectively. The most common G/P combination identified was G3P [8].

CONCLUSION

Group A rotaviruses was a major pathogen of diarrhea in Children in Lulong. G3P [8] was the predominant type in 2009, Gmix and Pmix abound, and G9 serotypes has become the second predominant after G3 strain in the region.

摘要

目的

分析河北省卢龙县轮状病毒腹泻的流行病学特征。

方法

收集426例5岁以下急性腹泻住院患儿的粪便标本。采用ELISA试剂盒鉴定轮状病毒阳性标本。多重半巢式RT-PCR法进行G/P分型检测。

结果

426份标本中202份（47.42%）检测出轮状病毒。轮状病毒基因分型显示，G3型占主导（57.9%），其次是G混合型（16.3%）、G9型（14.9%）、G1型（7.9%）、G4型（1%）、G2型（0.5%）；P基因分型显示，P[8]、P混合型、P[4]、P[9]型分别占58.4%、28.7%、6.9%和1%。最常见的G/P组合是G3P[8]。