Cheng Bin, Shi Hong, Ji Chang-fu, Li Jiang-hui, Chen Shu-li, Jing Xiang-hong
Shandong University of Chinese Medicine, Jinan 250355, China.
Zhen Ci Yan Jiu. 2010 Jun;35(3):193-7.
To observe the dynamic distribution of the extravasated Evans Blue (EB) dye points (neurogenic inflammatory response) at the skin after acute gastric mucosal injury (AGMI) and its relation to the related regular acupoints in the locations in rats.
A total of 70 Wistar rats were randomized into normal control (n=10), normal saline (n=10), and AGMI (n=50) groups. The AGMI group was further divided into 5 h, 2 d, 3 d, 4 d and 5 d subgroups with 10 rats in each. AGMI model was duplicated by intragastric perfusion of diluted hydrochloric acid (HCl, 0.5 mol/L). Evans Blue Dye (50 mg/kg, 50 mg/mL in 0.9% saline) was given to the rats before AGMI modeling. The plasma extravasated EB points at the skin of the whole body were observed after removal of the hair.
The extravasated EB points presented a nerve-segmental distribution, with the proportion of the points in the location being 47.5% for "Geshu" (BL 17),58. 82% for "Jizhong" (GV 6), 88.23% for "Pishu" (BL 20), 82.35% for "Weishu" (BL21), 17.64% for "Zhongwan" (CV 12), and 5.88% for "Shangwan" (CV 13), respectively. The plasma extravasation of EB seldom appeared in normal rats and only fewer points were found in rats accepted administration of 0.9% saline. Significant differences were found between model and normal control groups, and between model and normal saline groups in the numbers of the extravasated EB points (P < 0.01, P < 0.05). The number of the extravasated EB points was related to the phase of gastric mucosa injury, being most on the 2nd and 3rd day after modeling and disappearing gradually along with the natural repair of AGMI.
AGMI promotes the plasma extravasation of EB and the extravasated EB points present a nerve-segmental distribution and have a higher corresponding rate with some acupoints including "Pishu" (BL 20), "Weishu" (BL 21), etc., suggesting an activation of the normally silent acupoints under diseased conditions.
观察急性胃黏膜损伤（AGMI）后大鼠皮肤伊文思蓝（EB）染料渗出点（神经源性炎症反应）的动态分布及其与相关经穴的关系。
将70只Wistar大鼠随机分为正常对照组（n = 10）、生理盐水组（n = 10）和AGMI组（n = 50）。AGMI组再分为5 h、2 d、3 d、4 d和5 d亚组，每组10只。通过胃内灌注稀释盐酸（HCl，0.5 mol/L）复制AGMI模型。在AGMI建模前给大鼠注射伊文思蓝染料（50 mg/kg，溶于0.9%生理盐水中，浓度为50 mg/mL）。脱毛后观察全身皮肤血浆渗出的EB点。
EB渗出点呈神经节段性分布，各经穴部位的点数比例分别为：“膈俞”（BL 17）占47.5%，“脊中”（GV 6）占58.82%，“脾俞”（BL 20）占88.23%，“胃俞”（BL 21）占82.35%，“中脘”（CV 12）占17.64%，“上脘”（CV 13）占5.88%。正常大鼠很少出现EB血浆渗出，生理盐水组大鼠仅发现较少的点数。模型组与正常对照组、模型组与生理盐水组在EB渗出点数上有显著差异（P < 0.01，P < 0.05）。EB渗出点数与胃黏膜损伤阶段有关，建模后第2天和第3天最多，随着AGMI的自然修复逐渐消失。
AGMI促进EB血浆渗出，EB渗出点呈神经节段性分布，与“脾俞”（BL 20）、“胃俞”（BL 21）等部分经穴有较高的对应率，提示在疾病状态下正常沉默的经穴被激活。
