Yang Liqiang, Tang Lili, Liu Lan, Salam Nimaichand, Li Wen-Jun, Zhang Yongyu
Research Center for Marine Biology and Carbon Sequestration, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, People's Republic of China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Curr Microbiol. 2017 Aug;74(8):972-978. doi: 10.1007/s00284-017-1275-1. Epub 2017 Jun 6.
A gram-staining negative, non-motile, aerobic chemoheterotrophic, ovoid or short rod-shaped bacterium, designated as J89, was isolated from a seawater sample collected from the coast of Yellow Sea in Qingdao, China. The strain grew at salinities of 1.0-6.0% (w/v) NaCl (optimum, 3.0%). Growth occurred at pH 6.0-9.0 (optimum, pH 7.0) and at 10-35 °C (optimum, 25-30 °C). The genomic DNA G+C content was determined to be 59.3 mol%. Q-8 was detected as the respiratory quinone. The major fatty acids (>10%) were summed feature 3 (C ω7c and/or C ω6c), summed feature 8 (C ω7c and/or C ω6c), and C. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified phospholipids, and an unidentified polar lipid. Comparison of the 16S rRNA gene sequence indicated that the strain was most closely related (<91%) to members of the order Chromatiales in the class Gammaproteobacteria. Phylogenetic analyses showed that this strain represented a distinct phylogenetic lineage in the order Chromatiales and could not be assigned to any of the defined families in the order. On the basis of low sequence similarities and differential characteristics of strain J89 from the genera of neighboring families, the strain is proposed to be a representative of a novel genus Aquichromatium gen. nov. A new family Aquichromatiaceae with the type genus Aquichromatium is proposed. Strain J89 (=MCCC 1K03281T=CMRC C2017206T) is the type strain of the type species Aquichromatium aeriopus sp. nov.
从中国青岛黄海海岸采集的海水样本中分离出一株革兰氏染色阴性、无运动性、需氧化能异养型、卵形或短杆状细菌，命名为J89。该菌株在1.0 - 6.0%（w/v）NaCl盐度下生长（最适盐度为3.0%）。在pH 6.0 - 9.0（最适pH 7.0）和10 - 35°C（最适温度25 - 30°C）下生长。基因组DNA的G + C含量测定为59.3 mol%。检测到呼吸醌为Q - 8。主要脂肪酸（>10%）为总和特征3（C ω7c和/或C ω6c）、总和特征8（C ω7c和/或C ω6c）以及C。极性脂质由双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、两种未鉴定的磷脂和一种未鉴定的极性脂质组成。16S rRNA基因序列比较表明，该菌株与γ-变形菌纲色杆菌目的成员关系最为密切（<91%）。系统发育分析表明，该菌株代表色杆菌目中一个独特的系统发育谱系，不能归入该目中任何已定义的科。基于菌株J89与相邻科属的低序列相似性和差异特征，该菌株被提议为一个新属——水生色菌属（Aquichromatium gen. nov.）的代表。提出了一个以水生色菌属为模式属的新科——水生色菌科（Aquichromatiaceae）。菌株J89（=MCCC 1K03281T=CMRC C2017206T）是新物种气生水生色菌（Aquichromatium aeriopus sp. nov.）的模式菌株。
