Department of Biotechnology, College of Life Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu, Shiga 525-8577, Japan.
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Int J Syst Evol Microbiol. 2011 Aug;61(Pt 8):1973-1980. doi: 10.1099/ijs.0.026625-0. Epub 2010 Sep 17.
A Gram-negative, non-spore-forming, ovoid to rod-shaped aerobic or microaerobic bacterium, strain 262-8(T), was isolated from a cavity within white rock collected in Antarctica. Strain 262-8(T) grew at 5-30 °C (optimum 25 °C), at pH 6-8 (optimum approximately pH 7) and with 0.1-2.0 % (w/v) NaCl (optimum 0.5 % NaCl). The addition of tryptone or yeast extract was essential for growth. Strain 262-8(T) was able to utilize organic compounds such as ribose, pyruvate and succinate in the presence of a low concentration of tryptone. Ubiquinone 10 was the major respiratory quinone. The major fatty acids were C(18 : 1), C(16 : 0) and C(18 : 0). The G+C content of the genomic DNA was 69.8 mol%. Comparative analyses of 16S rRNA gene sequences and physiological characteristics indicated that strain 262-8(T) was a phylogenetically novel bacterium that should be classified in a new genus of the family Rhodospirillaceae, for which the name Constrictibacter antarcticus gen. nov., sp. nov. is proposed. The type strain of the type species is 262-8(T) ( = JCM 16422(T) = ATCC BAA-1906(T)).
一株革兰氏阴性、非芽孢形成、卵形到杆状需氧或微需氧细菌,菌株 262-8(T),从南极洲采集的白色岩石内部的腔室中分离得到。菌株 262-8(T)在 5-30°C(最佳 25°C)、pH 6-8(最佳约 pH 7)和 0.1-2.0%(w/v)NaCl(最佳 0.5%NaCl)的条件下生长。添加胰蛋白胨或酵母提取物对生长至关重要。在低浓度胰蛋白胨存在的情况下,菌株 262-8(T)能够利用核糖、丙酮酸和琥珀酸盐等有机化合物。泛醌 10 是主要的呼吸醌。主要脂肪酸为 C(18:1)、C(16:0)和 C(18:0)。基因组 DNA 的 G+C 含量为 69.8mol%。16S rRNA 基因序列和生理特性的比较分析表明,菌株 262-8(T)是一种系统发育上新颖的细菌,应归类于红螺旋菌科的一个新属,建议将其命名为南极收缩菌属(Constrictibacter),新种,南极收缩菌(Constrictibacter antarcticus)。模式种的模式菌株为 262-8(T)(=JCM 16422(T)=ATCC BAA-1906(T))。