1CAS Key Laboratory of Marine Bio-resources and Ecology; Guangdong Key Laboratory of Marine Materia Medica; RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, Guangdong 510301, PR China.
2University of Chinese Academy of Sciences, Beijing 100049, PR China.
Int J Syst Evol Microbiol. 2018 Dec;68(12):3707-3712. doi: 10.1099/ijsem.0.003016. Epub 2018 Oct 11.
A novel basophilic bacterial strain, designated as SCSIO 08040, was recovered from a deep-sea sediment sample collected from the Indian Ocean. The strain was Gram-stain-negative, vibrioid or spiral, light pink, 0.6-1.0 µm wide and 1.0-2.5 µm long. Growth occurred at 20-45 °C, pH 7-11 and <5 % (w/v) NaCl, with optimum growth at 28-37 °C, pH 7 and 0-3 % (w/v) NaCl. Catalase-, oxidase and urease-positive, nitrate reduction-negative. Analysis of 16S rRNA gene sequencing revealed that strain SCSIO 08040 had the highest similarity of 95.3 % to Rhodocista pekingensis 3-p. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences showed that the novel isolate formed a distinct phylogenetic lineage in the family Rhodospirillaceae. The whole-cell hydrolysate contained meso-diaminopimelic acid, galactose, mannose and xylose. The total cellular fatty acid profile was dominated by C18:1ω7c and C19:0cycloω8c. Q-10 was the predominant ubiquinone. The major phospholipids were diphosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine. The DNA G+C content of strain SCSIO 08040 was 66.82 mol%. Based on these polyphasic data, a new genus, Indioceanicola gen. nov., is proposed in the family Rhodospirillaceae with the type species Indioceanicola profundi sp. nov. and the type strain SCSIO 08040 (=DSM 105146=CGMCC 1.15812).
从印度洋深海沉积物样本中分离到一株革兰氏阴性、弧菌样或螺旋形、浅粉色的细菌菌株，命名为 SCSIO 08040。该菌株宽 0.6-1.0 μm，长 1.0-2.5 μm。在 20-45°C、pH7-11 和 <5%（w/v）NaCl 条件下生长，最适生长温度为 28-37°C、pH7 和 0-3%（w/v）NaCl。菌株 SCSIO 08040 接触酶、氧化酶和脲酶阳性，硝酸盐还原阴性。16S rRNA 基因序列分析表明，该菌株与 Rhodocista pekingensis 3-p 的相似性最高，为 95.3%。基于近全 16S rRNA 基因序列的系统发育分析表明，该新分离株在红螺菌科中形成了一个独特的进化分支。全细胞水解物含有 meso-二氨基庚二酸、半乳糖、甘露糖和木糖。总细胞脂肪酸组成以 C18:1ω7c 和 C19:0cycloω8c 为主。Q-10 是主要的泛醌。主要磷脂为双磷脂酰甘油、磷脂酰胆碱和磷脂酰乙醇胺。菌株 SCSIO 08040 的 DNA G+C 含量为 66.82 mol%。基于这些多相数据，建议在红螺菌科中建立一个新属，即 Indioceanicola 属，其模式种为深海 Indioceanicola profundi sp. nov.，模式菌株为 SCSIO 08040（=DSM 105146=CGMCC 1.15812）。
