School of Biological Science and Technology, University of Jinan, 250022, Jinan, PR China.
College of Marine Science, Shandong University, 264209, Weihai, PR China.
Arch Microbiol. 2021 Aug;203(6):3007-3013. doi: 10.1007/s00203-021-02286-7. Epub 2021 Mar 28.
A gram-stain-negative, non-motile and rod-shaped strain, designated wg1, was isolated from activated sludge obtained from wastewater treatment plant in Binzhou (Shandong province, PR China). Growth of strain wg1 occurred at 25-45 °C (optimum, 37 °C), at pH 7.0-9.0 (optimum growth at pH 8.0) and at a salinity range of 0-4% (optimum, 1%). The chemotaxonomic, phenotypic and genomic traits were investigated. The 16S rRNA gene sequence analysis showed that strain wg1 belonged to the genus Paracoccus. The species with highest similarity to strain wg1 was Paracoccus communis VKM B-2787 (98.27%), followed by Paracoccus kondratievae VKM B-2222 (98.25%). The isoprenoid quinone was Q-10. Major cellular fatty acids were summed feature 8, C and C. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), aminoglycolipid (AGL), phosphatidylglycerol (PG), phosphatidylcholine (PC), aminolipid (AL), one unidentified lipid (L) and one unidentified phospholipid (PL). The genome size was 4,834,448 bp with a G+C content of 67.67 mol%. The prediction result of secondary metabolites based on genome has shown that the strain wg1 contained 12 clusters, and the gene involved in primary metabolism showed differences in the comparison between wg1 and reference strains. The dDDH values of strain wg1 with P. communis VKM B-2787, P. kondratievae VKM B-2222 and P. denitrificans DSM 413 were 45.30, 30.60 and 39.50%, respectively. Based on its physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain wg1 is considered to represent a novel species for which the name Paracoccus binzhouensis sp. nov., is proposed. The type strain is wg1 (= KCTC 72861 = CCTCC AB 2019400).
从中国山东省滨州市污水处理厂的活性污泥中分离到一株革兰氏阴性、非运动和杆状的菌株，命名为 wg1。菌株 wg1 的生长温度为 25-45°C（最适温度为 37°C），pH 值为 7.0-9.0（最适生长 pH 值为 8.0），盐度范围为 0-4%（最适盐度为 1%）。对其进行了化学生态、表型和基因组特征的研究。16S rRNA 基因序列分析表明，菌株 wg1 属于副球菌属。与菌株 wg1 相似度最高的物种是副球菌属的 Paracoccus communis VKM B-2787（98.27%），其次是 Paracoccus kondratievae VKM B-2222（98.25%）。其类异戊二烯醌为 Q-10。主要的细胞脂肪酸为 summed feature 8、C 和 C。主要的极性脂为双磷脂酰甘油（DPG）、磷脂酰乙醇胺（PE）、氨基糖脂（AGL）、磷脂酰甘油（PG）、磷脂酰胆碱（PC）、氨基脂（AL）、一种未鉴定的脂（L）和一种未鉴定的磷脂（PL）。基因组大小为 4834488bp，G+C 含量为 67.67mol%。基于基因组的次生代谢产物预测结果表明，该菌株 wg1 含有 12 个簇，并且在与参考菌株的比较中，参与初级代谢的基因存在差异。wg1 与 Paracoccus communis VKM B-2787、Paracoccus kondratievae VKM B-2222 和 Paracoccus denitrificans DSM 413 的 dDDH 值分别为 45.30、30.60 和 39.50%。根据其生理特性、化学生态特征以及较低的 ANI 和 dDDH 结果，菌株 wg1 被认为代表一个新的物种，被命名为 Paracoccus binzhouensis sp. nov.。其模式菌株为 wg1（=KCTC 72861=CCTCC AB 2019400）。
