Department of Bio Technology, Division of Environmental & Life Science, College of Natural Science, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon 433-760, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1311-1316. doi: 10.1099/ijs.0.035212-0. Epub 2012 Jul 13.
Two strains of gram-negative, catalase- and oxidase-positive, coccus-shaped bacteria, designated NB88(T) and LNB004, were isolated from activated sludge in the Mae-san sewage treatment plant in South Korea. They were characterized in a polyphasic taxonomic study based on phenotypic, phylogenetic and genotypic approaches. Comparative 16S rRNA gene sequence analysis indicated that strains NB88(T) and LNB004 represented a novel subline within the genus Paracoccus in the family Rhodobacteraceae. According to 16S rRNA gene sequence comparisons, strains NB88(T) and LNB004 were indistinguishable and showed 94.5-97.6 % similarity to the type strains of other Paracoccus species. Strain NB88(T) exhibited relatively high levels of DNA hybridization (84±3.5 %) with LNB004 and low hybridization values (<40 %) with type strains of other Paracoccus species. Both strains showed chemotaxonomic characteristics typical of the genus Paracoccus, with Q-10 as the predominant respiratory quinone and C18 : 1ω7c as the major fatty acid, and both strains accumulated poly-β-hydroxybutyrate granules. The DNA G+C contents of strains NB88(T) and LNB004 were 66.4 and 65.1 mol%, respectively. The polar lipid profiles of strains NB88(T) and LNB004 included major amounts of phosphatidylglycerol, phosphatidylcholine and an unknown aminolipid. The taxonomic position of strains NB88(T) and LNB004 was clarified by the low level of DNA-DNA hybridization with closely related strains and the strains could be distinguished from other recognized species by using biochemical tests and molecular genetic analysis. On the basis of their phenotypic and genotypic properties and their phylogenetic distinctiveness, strains NB88(T) and LNB004 should be classified in a novel species of the genus Paracoccus, for which the name Paracoccus limosus sp. nov. is proposed. The type strain is NB88(T) ( = KEMC 5401-184(T) = JCM 17370(T)); strain LNB004 ( = KEMC 5401-001) is a reference strain.
两株革兰氏阴性、过氧化氢酶和氧化酶阳性、球菌形状的细菌，分别命名为 NB88(T) 和 LNB004，从韩国 Mae-san 污水处理厂的活性污泥中分离得到。它们的特征是基于表型、系统发育和基因型方法的多相分类研究。比较 16S rRNA 基因序列分析表明，NB88(T)和 LNB004 株代表了红杆菌科 Paracoccus 属中的一个新亚群。根据 16S rRNA 基因序列比较，NB88(T)和 LNB004 株与其他 Paracoccus 种的模式株相似度为 94.5-97.6%。NB88(T)株与 LNB004 株的 DNA 杂交水平相对较高（84±3.5%），与其他 Paracoccus 种的模式株杂交值较低（<40%）。两株菌均表现出典型的 Paracoccus 属化感特征，以 Q-10 为主要呼吸醌，C18：1ω7c 为主要脂肪酸，两株菌均积累多-β-羟基丁酸颗粒。NB88(T)和 LNB004 株的 DNA G+C 含量分别为 66.4 和 65.1mol%。NB88(T)和 LNB004 株的极性脂谱包括大量的磷脂酰甘油、磷脂酰胆碱和一种未知的氨基脂。NB88(T)和 LNB004 株与亲缘关系密切的菌株的 DNA-DNA 杂交水平较低，通过生化试验和分子遗传学分析可将其与其他公认的种区分开来，从而明确了它们的分类地位。基于表型和基因型特征以及系统发育上的独特性，NB88(T)和 LNB004 株应归类于 Paracoccus 属的一个新种，命名为 Paracoccus limosus sp. nov.。模式株为 NB88(T)（=KEMC 5401-184(T)=JCM 17370(T)）；菌株 LNB004（=KEMC 5401-001）为参考株。
