一种用于检测临床标本中曲霉菌种DNA的巢式定性实时PCR检测方法的开发。
Development of a nested qualitative real-time PCR assay to detect Aspergillus species DNA in clinical specimens.
作者信息
Halliday Catriona, Wu Qi Xuan, James Gregory, Sorrell Tania
机构信息
NHMRC Clinical Center of Research Excellence in Infectious Diseases, Hematology, and Health Ethics, University of Sydney Western Clinical School, New South Wales 2145, Australia.
出版信息
J Clin Microbiol. 2005 Oct;43(10):5366-8. doi: 10.1128/JCM.43.10.5366-5368.2005.
Abstract
We adapted a nested Aspergillus PCR to a nested qualitative real-time format on a Light Cycler system. An evaluation using 134 clinical specimens showed that the real-time PCR assay significantly reduced the time for results to be made available without compromising sensitivity and was less labor-intensive. Its use will aid in the rapid diagnosis of invasive aspergillosis.
摘要
我们将巢式曲霉PCR方法调整为适用于Light Cycler系统的巢式定性实时检测方法。对134份临床标本进行的评估显示，实时PCR检测在不影响灵敏度的情况下显著缩短了出结果的时间，且劳动强度更低。其应用将有助于侵袭性曲霉病的快速诊断。
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