Morris D D, Crowe N, Moore J N
Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602.
Am J Vet Res. 1990 Dec;51(12):1935-40.
Serum tumor necrosis factor (TNF) activity was quantitated in 8 horses given an IV infusion of endotoxin (0.03 micrograms of lipopolysaccharide/kg of body weight, from Escherichia coli 055:B5) in 0.9% NaCl solution over 1 hour. Serum TNF activity was likewise measured in 6 horses given only 0.9% sterile NaCl solution at the same rate. The duration of serum TNF activity was determined, and serum TNF activity was correlated with clinical and laboratory changes during the induced endotoxemia. Horses had no serum TNF activity prior to endotoxin administration, but geometric mean serum TNF activity was significantly higher from 1 to 4 hours after the start of the infusion. In response to endotoxin, horses seemed depressed, had signs of mild to moderate abdominal pain, developed tachycardia and fever, and had leukopenia followed by leukocytosis. Association between serum TNF activity and temperature, heart rate, attitude abnormality score, and WBC count of horses given endotoxin was significant. Serum TNF activity had a significant positive linear correlation with attitude abnormality and heart rate and a negative linear correlation with the WBC count during endotoxemia. Geometric mean serum TNF activity peaked approximately 1.5 hours prior to mean peak fever, and these data were significantly correlated. Results of this study suggest that TNF is an important mediator of endotoxemia in horses.
对8匹马静脉输注内毒素（0.03微克脂多糖/千克体重，来源于大肠杆菌055:B5），溶于0.9%氯化钠溶液中，在1小时内输完，测定其血清肿瘤坏死因子（TNF）活性。同样以相同速率对6匹马仅输注0.9%无菌氯化钠溶液，测定其血清TNF活性。确定血清TNF活性的持续时间，并将血清TNF活性与诱导内毒素血症期间的临床和实验室变化相关联。在内毒素给药前，马没有血清TNF活性，但在输注开始后1至4小时，血清TNF活性的几何平均值显著更高。对内毒素的反应中，马表现出抑郁，有轻度至中度腹痛的体征，出现心动过速和发热，并有白细胞减少随后白细胞增多。内毒素处理马的血清TNF活性与体温、心率、态度异常评分和白细胞计数之间存在显著关联。在内毒素血症期间，血清TNF活性与态度异常和心率呈显著正线性相关，与白细胞计数呈负线性相关。血清TNF活性的几何平均值在平均发热峰值前约1.5小时达到峰值，且这些数据显著相关。本研究结果表明，TNF是马内毒素血症的重要介质。
