Kazemi Arababadi Mohammad
Department of Microbiology, Hematology, and Immunology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Iran J Kidney Dis. 2010 Oct;4(4):302-6.
INTRODUCTION. The impact of several environmental and genetic factors on diabetes mellitus and its complications is well documented. It has also been established that cytokines play key roles in the pathogenesis of nephropathy. Polymorphisms of the -590 region of interleukin (IL)-4 are associated with the regulation of expression of this gene. In this study, we aimed to find polymorphisms of this region in nephropathic type 2 diabetic patients. MATERIALS AND METHODS. Peripheral blood samples were collected from 100 type 2 diabetic patients with nephropathy and 150 healthy controls. DNA was extracted and a polymerase chain reaction-restricted fragment length polymorphism technique was performed to examine polymorphisms in the -590 region of the IL-4 gene. RESULTS. Our results showed a significant difference between the C/C, T/C, and T/T genotypes and the C and T alleles of the -590 region of IL-4 in nephropathic patients in comparison with the healthy controls. CONCLUSIONS. Results of this study suggest that the functional gene polymorphisms of IL-4 play an important role in the pathogenesis of diabetic nephropathy in patients with type 2 diabetes mellitus.
引言。多种环境和遗传因素对糖尿病及其并发症的影响已有充分记录。细胞因子在肾病发病机制中起关键作用也已得到证实。白细胞介素（IL）-4基因-590区域的多态性与该基因的表达调控相关。在本研究中，我们旨在寻找2型糖尿病肾病患者该区域的多态性。材料与方法。从100例2型糖尿病肾病患者和150例健康对照者中采集外周血样本。提取DNA，并采用聚合酶链反应-限制性片段长度多态性技术检测IL-4基因-590区域的多态性。结果。我们的结果显示，与健康对照相比，肾病患者IL-4基因-590区域的C/C、T/C和T/T基因型以及C和T等位基因存在显著差异。结论。本研究结果表明，IL-4的功能性基因多态性在2型糖尿病患者糖尿病肾病的发病机制中起重要作用。
