APN/CD13 和 NEP/CD10 对精子活力的控制。
Control of APN/CD13 and NEP/CD10 on sperm motility.
机构信息
Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country, Leioa, Bizkaia 48940, Spain.
出版信息
Asian J Androl. 2010 Nov;12(6):899-902. doi: 10.1038/aja.2010.82. Epub 2010 Sep 20.
Aminopeptidase N (APN/CD13) and neutral endopeptidase (NEP/CD10) are enzymes present in human sperm cells and involved in regulation of sperm motility of noncapacitated spermatozoa. We investigated the involvement of APN/CD13 and NEP/CD10 in motility and in kinematic parameters of human capacitated spermatozoa. Sperm cells isolated by a discontinuous Percoll gradient (40%-80%) followed up by swim-up techniques were incubated with the APN/CD13-specific inhibitor, leuhistin (100 μmol L(-1)), and the NEP/CD10-specific inhibitor, thiorphan (1 μmol L(-1)). The complete inhibition of both APN/CD13 and NEP/CD10 improved sperm motility. Spermatozoa incubated with the APN/CD13-specific inhibitor leuhistin showed asymmetrical trajectories, whereas sperm trajectories were more regular after treatment with the NEP/CD10-specific inhibitor thiorphan. In conclusion, APN/CD13 and NEP/CD10 modulate the motility of capacitated spermatozoa, although each of the enzymes seems to participate in the control of different aspects of sperm motility. Therefore, both inhibitors may be useful for sperm activation at different functional stages of spermatozoa.
氨基肽酶 N（APN/CD13）和中性内肽酶（NEP/CD10）是存在于人类精子细胞中的酶，参与调节非获能精子的运动能力。我们研究了 APN/CD13 和 NEP/CD10 在人获能精子运动能力和运动学参数中的作用。通过不连续 Percoll 梯度（40%-80%）分离的精子细胞，然后通过游泳技术进行培养，并用 APN/CD13 特异性抑制剂亮抑酶肽（100 μmol/L）和 NEP/CD10 特异性抑制剂硫普罗宁（1 μmol/L）进行孵育。两种酶 APN/CD13 和 NEP/CD10 的完全抑制均可改善精子的运动能力。用 APN/CD13 特异性抑制剂亮抑酶肽孵育的精子表现出不对称轨迹，而在用 NEP/CD10 特异性抑制剂硫普罗宁处理后，精子轨迹更加规则。总之，APN/CD13 和 NEP/CD10 调节获能精子的运动能力，尽管每种酶似乎都参与了对精子运动能力的不同方面的控制。因此，两种抑制剂可能在精子的不同功能阶段对精子激活有用。
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