Linder P, Prat A
Department of Microbiology, Biozentrum, Basel, Switzerland.
Bioessays. 1990 Nov;12(11):519-26. doi: 10.1002/bies.950121103.
The possibility of combining powerful genetic methods with biochemical analysis has made baker's yeast Saccharomyces cerevisiae the organism of choice to study the complex process of translation initiation in eukaryotes. Several new initiation factor genes and interactions between components of the translational machinery that were not predicted by current models have been revealed by genetic analysis of extragenic suppressors of translational initiation mutants. In addition, a yeast cell-free translation system has been developed that allows in vivo phenotypes to be correlated with in vitro biochemical activities. We summarize here the current view of yeast translational initiation obtained by these approaches.
将强大的遗传学方法与生化分析相结合的可能性，使得面包酵母酿酒酵母成为研究真核生物翻译起始复杂过程的首选生物体。通过对翻译起始突变体的基因外抑制子进行遗传分析，揭示了几个新的起始因子基因以及当前模型未预测到的翻译机制组件之间的相互作用。此外，还开发了一种酵母无细胞翻译系统，该系统可使体内表型与体外生化活性相关联。我们在此总结通过这些方法获得的关于酵母翻译起始的当前观点。
