Romanova E A, Oretskaia T S, Sukhomlinov V V, Krynetskaia N F, Metelev V G, Shabarova Z A
Bioorg Khim. 1990 Oct;16(10):1348-54.
Several oligo(ribodeoxyribo)nucleotides to be used as probes in the RNase H-induced hydrolyses of 5S ribosomal RNA E. coli were synthesized on the Victoria-4M automatic gene synthesizer by the phosphoramidite approach, which allows for introducing ribonucleotides into any position of the oligomer. 2'-Hydroxy function was protected by tert-butyldimethylsilyl group whose hydrophobicity simplified isolation of the oligonucleotides by reverse-phased HPLC.
几种用作核糖核酸酶H诱导大肠杆菌5S核糖体RNA水解反应探针的寡聚（核糖脱氧核糖）核苷酸，采用亚磷酰胺法在Victoria-4M自动基因合成仪上合成，该方法可将核糖核苷酸引入寡聚物的任何位置。2'-羟基功能由叔丁基二甲基硅烷基保护，其疏水性通过反相高效液相色谱法简化了寡核苷酸的分离。
