Zaiakina G V, Krynetskaia N F, Metelev V G, Shabarova Z A
Bioorg Khim. 1987 Feb;13(2):266-8.
Oligo (ribodeoxyribo)nucleotide d(TGTGTAT)r(GCCAU) was synthesized from oligonucleotide blocks by chemical ligation, the oligoribonucleotide and oligodeoxynucleotide blocks being prepared by enzymatic and chemical synthesis, resp. A general procedure of the hybride duplex strand separation is described.
寡聚(核糖脱氧核糖)核苷酸d(TGTGTAT)r(GCCAU) 通过化学连接从寡核苷酸片段合成,寡核糖核苷酸片段和寡脱氧核糖核苷酸片段分别通过酶促合成和化学合成制备。描述了杂交双链链分离的一般方法。