Yi Lixin, Bi Kaishun, Chen Xinyang, Zhang Qiufeng, Che Shuang, Liu Wentao, Lu Dan, Chen Xiaohui
School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, Liaoning 110016, People's Republic of China.
Biomed Chromatogr. 2010 Oct;24(10):1089-93. doi: 10.1002/bmc.1409.
A simple and sensitive LC-MS method for the determination of periplocin in rat plasma was developed and validated. The chromatographic separation was carried out using a reverse-phase Kromasil C(18) column(150 × 4.6 mm, i.d., 5 µm) with a mobile phase composed of methanol-water (76:24, v/v). The flow rate of mobile phase was 0.8 mL/min. The calibration curve was linear within the concentration range 1-1000 ng/mL. The intra- and inter-day precisions across three validation days over the entire concentration range was lower than 9.2% in terms of relative standard deviation. Accuracy determined at three quality control concentrations ranged from -2.0 to 6.0% in terms of relative error. The validated method was applied to the pharmacokinetic study of periplocin in rat plasma after intravenous and intramuscular administration.
