Mikami K, Sato S, Watanabe T
Department of Legal Medicine, Nippon Medical School, Tokyo, Japan.
Alcohol Alcohol. 1990;25(6):651-60. doi: 10.1093/oxfordjournals.alcalc.a045062.
Myocardial cells in monolayer culture were examined ultrastructurally at various times (5 min-24 hr) after addition of ethanol (12.5-500 mM). At 5 min, all concentrations of ethanol induced mitochondrial fusion and a remarkable increase in glycogen granules, and the mitochondrial outer membrane seemed more fragile. These initial ultrastructural changes then recovered with time. Glycogen granules were decreased, then returned to normal more rapidly as the ethanol concentration was increased. Mitochondrial fusion and fragile appearance of the outer membrane were hardly seen at 1 hr in the presence of 12.5 or 50 mM ethanol, but were still evident with higher ethanol concentrations (200 and 500 mM), though less frequently than at 5 and 30 min. Furthermore, 'giant mitochondria' (with at least a five-fold increase in sectional area) appeared in the presence of 200 and 500 mM ethanol with increasing time of exposure. These results are discussed in relation to the direct effects of ethanol.
在添加乙醇（12.5 - 500 mM）后的不同时间点（5分钟 - 24小时），对单层培养的心肌细胞进行超微结构检查。在5分钟时，所有浓度的乙醇均诱导线粒体融合和糖原颗粒显著增加，并且线粒体外膜似乎更脆弱。这些初始的超微结构变化随后随时间恢复。糖原颗粒减少，然后随着乙醇浓度的增加更快地恢复正常。在存在12.5或50 mM乙醇的情况下，1小时时几乎看不到线粒体融合和外膜的脆弱外观，但在较高乙醇浓度（200和500 mM）下仍很明显，尽管比5分钟和30分钟时出现的频率低。此外，随着暴露时间的增加，在存在200和500 mM乙醇的情况下出现了“巨型线粒体”（横截面积至少增加五倍）。结合乙醇的直接作用对这些结果进行了讨论。
