富含番茄红素的番茄酱可预防人体皮肤光损伤：一项随机对照试验。

Tomato paste rich in lycopene protects against cutaneous photodamage in humans in vivo: a randomized controlled trial.

机构信息

Dermatological Sciences, Epithelial Sciences Research Group, The University of Manchester and Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester M6 8HD, UK.

出版信息

Br J Dermatol. 2011 Jan;164(1):154-62. doi: 10.1111/j.1365-2133.2010.10057.x. Epub 2010 Nov 29.

DOI:10.1111/j.1365-2133.2010.10057.x

PMID:20854436

Abstract

BACKGROUND

Previous epidemiological, animal and human data report that lycopene has a protective effect against ultraviolet radiation (UVR)-induced erythema.

OBJECTIVES

We examined whether tomato paste--rich in lycopene, a powerful antioxidant--can protect human skin against UVR-induced effects partially mediated by oxidative stress, i.e. erythema, matrix changes and mitochondrial DNA (mtDNA) damage.

METHODS

In a randomized controlled study, 20 healthy women (median age 33 years, range 21-47; phototype I/II) ingested 55 g tomato paste (16 mg lycopene) in olive oil, or olive oil alone, daily for 12 weeks. Pre- and postsupplementation, UVR erythemal sensitivity was assessed visually as the minimal erythema dose (MED) and quantified with a reflectance instrument. Biopsies were taken from unexposed and UVR-exposed (3 × MED 24 h earlier) buttock skin pre- and postsupplementation, and analysed immunohistochemically for procollagen (pC) I, fibrillin-1 and matrix metalloproteinase (MMP)-1, and by quantitative polymerase chain reaction for mtDNA 3895-bp deletion.

RESULTS

Mean ± SD erythemal D(30) was significantly higher following tomato paste vs. control (baseline, 26·5 ± 7·5 mJ cm(-2); control, 23 ± 6·6 mJ cm(-2); tomato paste, 36·6 ± 14·7 mJ cm(-2); P = 0·03), while the MED was not significantly different between groups (baseline, 35·1 ± 9·9 mJ cm(-2); control, 32·6 ± 9·6 mJ cm(-2); tomato paste, 42·2 ± 11·3 mJ cm(-2)). Presupplementation, UVR induced an increase in MMP-1 (P = 0·01) and a reduction in fibrillin-1 (P = 0·03). Postsupplementation, UVR-induced MMP-1 was reduced in the tomato paste vs. control group (P = 0·04), while the UVR-induced reduction in fibrillin-1 was similarly abrogated in both groups, and an increase in pCI deposition was seen following tomato paste (P = 0·05). mtDNA 3895-bp deletion following 3 × MED UVR was significantly reduced postsupplementation with tomato paste (P = 0·01).

CONCLUSIONS

Tomato paste containing lycopene provides protection against acute and potentially longer-term aspects of photodamage.

摘要

背景

先前的流行病学、动物和人体数据报告称，番茄红素对紫外线辐射（UVR）诱导的红斑具有保护作用。

目的

我们研究了富含番茄红素（一种强大的抗氧化剂）的番茄酱是否可以保护人体皮肤免受 UVR 诱导的部分氧化应激介导的影响，即红斑、基质变化和线粒体 DNA（mtDNA）损伤。

方法

在一项随机对照研究中，20 名健康女性（中位年龄 33 岁，范围 21-47；肤色 I/II）每日摄入 55 克番茄酱（含 16 毫克番茄红素）或橄榄油，持续 12 周。在补充前后，通过视觉评估最小红斑剂量（MED）和反射仪定量评估 UVR 红斑敏感性。在补充前后，从未暴露和 UVR 暴露（24 小时前接受 3×MED）的臀部皮肤中采集活检，并进行组织学分析以检测原胶原蛋白（pC）I、原纤维蛋白-1 和基质金属蛋白酶（MMP）-1，并通过定量聚合酶链反应检测 mtDNA 3895-bp 缺失。

结果

与对照组相比（基线，26.5±7.5mJ/cm(2)；对照组，23±6.6mJ/cm(2)；番茄酱组，36.6±14.7mJ/cm(2)；P=0.03），番茄酱组的红斑 D(30)明显更高，而 MED 组之间无显著差异（基线，35.1±9.9mJ/cm(2)；对照组，32.6±9.6mJ/cm(2)；番茄酱组，42.2±11.3mJ/cm(2)）。在补充前，UVR 诱导 MMP-1 增加（P=0.01），原纤维蛋白-1 减少（P=0.03）。补充后，番茄酱组的 UVR 诱导 MMP-1 减少（P=0.04），而两组的 UVR 诱导原纤维蛋白-1 减少相似，并且在番茄酱组中观察到 pCI 沉积增加（P=0.05）。在接受 3×MED UVR 后，mtDNA 3895-bp 缺失在补充番茄酱后明显减少（P=0.01）。