Delanghe Lize, De Boeck Ilke, Van Malderen Joke, Gehrmann Thies, Allonsius Camille Nina, Bron Peter A, Claes Ingmar, Hagendorens Margo, Leysen Julie, Wittouck Stijn, Lebeer Sarah
Department of Bioscience Engineering, University of Antwerp, Antwerpen 2020, Belgium.
YUN NV, Niel 2845, Belgium.
Microbiome Res Rep. 2024 Aug 29;3(4):43. doi: 10.20517/mrr.2024.23. eCollection 2024.
The human skin microbiome plays an essential role in protecting against pathogens and other external substances. This open ecosystem is also influenced by personal and environmental factors, but the precise impact of these factors, such as lifestyle and season, is understudied. We focused here on the inner elbow, a skin site prone to inflammatory conditions like atopic dermatitis and psoriasis. We collected skin swabs from the inner elbow of 52 children and adults, with no signs of skin disorders, in the winter and summer seasons. Samples were analyzed using metagenomic shallow shotgun sequencing. In addition, metadata were collected using questionnaires on health, lifestyle, and environmental factors. The core inner elbow community, taxa with a prevalence of 95% or higher, consisted of several well-known skin taxa, such as , , , and . In addition, and species were also found to be highly prevalent members of the skin microbiota, especially in the age group up to 3 years old. Of all investigated factors, age appeared to be the major driver defining the skin microbiome composition and longitudinal stability over the seasons. Differential abundance analysis using three statistical tests also pointed out that specific skin species were significantly associated with sampling season, age, hygiene practices, vitamin D supplements, probiotics, and the number of household members. This study identifies novel factors influencing the inner elbow skin microbiome composition and paves the way for future comparative and intervention studies in skin disorders such as atopic dermatitis.
人类皮肤微生物群落在抵御病原体和其他外部物质方面发挥着重要作用。这个开放的生态系统也受到个人和环境因素的影响，但这些因素，如生活方式和季节，的确切影响尚未得到充分研究。我们在此聚焦于肘内侧，这是一个易患特应性皮炎和银屑病等炎症性疾病的皮肤部位。我们在冬季和夏季从52名无皮肤疾病迹象的儿童和成人的肘内侧采集了皮肤拭子。样本采用宏基因组浅层鸟枪法测序进行分析。此外，还通过关于健康、生活方式和环境因素的问卷收集了元数据。肘内侧核心群落，即患病率达95%或更高的分类群，由几种知名的皮肤分类群组成，如 、 、 和 。此外， 和 物种也被发现是皮肤微生物群中高度普遍的成员，尤其是在3岁及以下年龄组。在所有调查因素中，年龄似乎是决定皮肤微生物群组成以及跨季节纵向稳定性的主要驱动因素。使用三种统计检验进行的差异丰度分析还指出，特定的皮肤物种与采样季节、年龄、卫生习惯、维生素D补充剂、益生菌以及家庭成员数量显著相关。这项研究确定了影响肘内侧皮肤微生物群组成的新因素，并为未来在特应性皮炎等皮肤疾病方面的比较和干预研究铺平了道路。
