Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo, 186-8650, Japan.
J Dairy Sci. 2010 Oct;93(10):4526-34. doi: 10.3168/jds.2010-3274.
Helicobacter pylori infection alters gene expression in host cells. Specifically, inflammatory chemokines such as IL-8 are upregulated in the gastric mucosa during H. pylori infection. Although the mechanism by which H. pylori causes inflammation of the gastric mucosa is not yet understood, many studies have suggested that nuclear factor kappa B (NF-κB) plays a key regulatory role in host cells. We have shown that preincubation with Bifidobacterium bifidum strain BF-1, a probiotic strain known to improve H. pylori-associated gastritis, suppresses induction of IL-8 by the pathogen. To investigate how how BF-1 affects gene expression in H. pylori-infected cells, we performed microarray analysis to assess gene expression in epithelial cells, which had been preincubated with BF-1 and infected with H. pylori. We found that preincubation with BF-1 suppresses the expression of H. pylori-induced genes in human cells and that most of the affected genes are related to the NF-κB signaling pathways. These results suggest that BF-1 can affect the regulatory mechanism of the NF-κB signaling pathways.
幽门螺杆菌感染会改变宿主细胞的基因表达。具体来说，在幽门螺杆菌感染期间，胃黏膜中的炎症趋化因子（如 IL-8）会被上调。尽管幽门螺杆菌引起胃黏膜炎症的机制尚不清楚，但许多研究表明，核因子 kappa B（NF-κB）在宿主细胞中发挥关键调节作用。我们已经表明，预先用双歧杆菌 BF-1 孵育，一种已知可改善与幽门螺杆菌相关的胃炎的益生菌株，可抑制病原体诱导的 IL-8 的产生。为了研究 BF-1 如何影响感染幽门螺杆菌的细胞中的基因表达，我们进行了微阵列分析，以评估预先用 BF-1 孵育并感染幽门螺杆菌的上皮细胞中的基因表达。我们发现，BF-1 的预先孵育可抑制人细胞中幽门螺杆菌诱导基因的表达，并且受影响的大多数基因与 NF-κB 信号通路有关。这些结果表明 BF-1 可以影响 NF-κB 信号通路的调节机制。
