Department of Entomology, Tree Fruit Research and Extension Center, Washington State University, 1100 N. Western Avenue, Wenatchee, WA 98801, USA.
J Econ Entomol. 2010 Aug;103(4):1378-85. doi: 10.1603/ec09354.
Obliquebanded leafroller, Choristoneura rosaceana (Harris) (Lepidoptera: Tortricidae), is a major pest of pome fruit in Washington. The use of broad-spectrum insecticides for decades has led to the development of insecticide resistance in C. rosaceana. Recently registered insecticides with novel modes of action, such as chlorantraniliprole and spinetoram, have provided effective C. rosaceana control, but resistance remains a threat. The risk of insecticide resistance development in a pest can be assessed by artificial selection in the laboratory. Subsequently, this information can be valuable in developing strategies to retain susceptibility in the field. A laboratory population of C. rosaceana was selected after repeated exposure to chlorantraniliprole and spinetoram to determine the risk of resistance evolution. After six generations of selection, 6.58- and 3.64-fold increases in LC50 were recorded for chlorantraniliprole and spinetoram, respectively. The realized heritability (h2) of resistance was estimated as 0.17 for chlorantraniliprole and 0.18 for spinetoram by using threshold trait analysis. The rates of resistance development were compared using the response quotient (Q), which was estimated as 0.11 and 0.07 for chlorantraniliprole and spinetoram, respectively. Projected rates of resistance evolution indicated that if h2 = 0.2 and 80% of the population was killed at each generation, then a 10-fold increase in LC50 would be expected in less than six generations for chlorantraniliprole and 10 generations for spinetoram. These results indicate that the risk of resistance development in C. rosaceana exists to both of these insecticides but that resistance development in C. rosaceana would be slower against spinetoram than chlorantraniliprole.
斜纹带卷叶蛾，Choristoneura rosaceana (Harris)（鳞翅目：卷蛾科），是华盛顿地区苹果果实的主要害虫。几十年来广谱杀虫剂的使用导致 C. rosaceana 产生了抗药性。最近注册的具有新型作用模式的杀虫剂，如氯虫苯甲酰胺和乙基多杀菌素，已提供了有效的 C. rosaceana 防治，但抗药性仍然是一个威胁。通过实验室人工选择，可以评估害虫产生抗药性的风险。随后，这一信息对于制定在田间保持敏感性的策略非常有价值。通过反复接触氯虫苯甲酰胺和乙基多杀菌素来选择 C. rosaceana 的实验室种群，以确定抗药性进化的风险。经过六代选择，氯虫苯甲酰胺和乙基多杀菌素的 LC50 分别增加了 6.58 倍和 3.64 倍。通过阈性状分析，氯虫苯甲酰胺和乙基多杀菌素的抗性现实遗传力（h2）分别估计为 0.17 和 0.18。使用反应商（Q）比较了抗性发展的速度，氯虫苯甲酰胺和乙基多杀菌素的 Q 分别估计为 0.11 和 0.07。抗性进化的预测速度表明，如果 h2 = 0.2，并且每代有 80%的种群被杀死，那么氯虫苯甲酰胺的 LC50 预计将在不到六代的时间内增加 10 倍，而乙基多杀菌素则需要 10 代。这些结果表明，C. rosaceana 对这两种杀虫剂都存在抗药性发展的风险，但 C. rosaceana 对乙基多杀菌素的抗药性发展速度将比氯虫苯甲酰胺慢。
