Ricart Arbona Rodolfo J, Lipman Neil S, Wolf Felix R
Tri-Institutional Training Program in Laboratory Animal Medicine and Science, Memorial Sloan-Kettering Cancer Center, Weill Cornell Medical College and The Rockefeller University, New York, New York, USA.
J Am Assoc Lab Anim Sci. 2010 Sep;49(5):583-7.
Fur mites are a persistent problem in contemporary laboratory mouse colonies. We conducted several studies to evaluate fur mite diagnostic methodologies and interpretation of results. Retrospective analysis of test results from sentinel mice exposed to soiled bedding collected from colonies infested with Myobia musculi and Myocoptes musculinus revealed the skin scrape test to be more reliable than pelt examination, provided that both the head and dorsal thoracolumbar regions were sampled. To assess their diagnostic accuracy, 3 commercial laboratories were sent positive control slides containing mites, mite parts, or eggs in sets of slides containing diagnostic skin scrapings in varying ratios. Laboratory B correctly identified the positive control slide. Laboratory A identified 1 of 3 positive control slides, whereas laboratory C failed to identify both positive control slides submitted. To determine the time required for a mouse to shed its entire hair coat, fur of Crl:CD1(ICR), BALB/cAnNCrl, and Crl:CFW(SW) albino mice was dyed black and the presence of dyed fur evaluated monthly for 8 mo. Limited dyed hair was still present at 8 mo; therefore, finding eggs or egg casings many months after treatment cessation does not necessarily imply treatment failure. To evaluate the effectiveness of soiled bedding sentinels for detection of fur mites in a mite-infested colony, we exposed naïve mice to varying amounts (100%, 50%, 25%, 2.5%, and 0%) of soiled bedding in clean bedding. As little as 2.5% soiled bedding resulted in detection of a positive sentinel within a 2-mo period.
皮螨是当代实验小鼠群体中一个长期存在的问题。我们进行了多项研究，以评估皮螨的诊断方法和结果解读。对暴露于感染了鼠肌螨和鼠疥螨的群体所收集的脏垫料中的哨兵小鼠的检测结果进行回顾性分析发现，只要对头和背侧胸腰椎区域都进行采样，皮肤刮片试验比皮毛检查更可靠。为评估其诊断准确性，向3家商业实验室发送了含有螨虫、螨虫部分或虫卵的阳性对照玻片，这些玻片与含有不同比例诊断性皮肤刮片的玻片放在一起。实验室B正确识别出阳性对照玻片。实验室A识别出了3张阳性对照玻片中的1张，而实验室C未能识别提交的两张阳性对照玻片。为确定小鼠蜕去其整个毛发覆盖所需的时间，将Crl:CD1(ICR)、BALB/cAnNCrl和Crl:CFW(SW)白化小鼠的毛发染成黑色，并在8个月内每月评估染色毛发的存在情况。8个月时仍有少量染色毛发；因此，在停止治疗数月后发现虫卵或卵壳并不一定意味着治疗失败。为评估脏垫料哨兵在检测螨虫感染群体中的皮螨方面的有效性，我们将未感染的小鼠暴露于干净垫料中不同量（100%、50%、25%、2.5%和0%）的脏垫料中。低至2.5%的脏垫料在2个月内就导致检测出一只阳性哨兵。
